Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Antibody Depletion, Bioassay, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TSLP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TSLP
Tyr29-Gln159
Accession # Q969D9

Specificity

Detects human TSLP in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse TSLP and recombinant human TSLP R is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TSLP Antibody

Cell Proliferation Induced by TSLP and Neutralization by Human TSLP Antibody.

Cell Proliferation Induced by TSLP and Neutralization by Human TSLP Antibody.

Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 Ra and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398). The ND50 is typically 0.05-0.25 µg/mL.

Applications for Human TSLP Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TSLP (Catalog # 1398-TS)

Neutralization

Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 0.5 ng/mL Recombinant Human TSLP.

Human TSLP Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human TSLP Biotinylated Antibody (Catalog # BAF1398)
  • Standard: Recombinant Human TSLP Protein (Catalog # 1398-TS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 3 using AF1398 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSLP

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis, and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)

Gene Symbol

TSLP

UniProt

Q969D9

Additional TSLP Products

Product Documents for Human TSLP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TSLP Antibody

For research use only

Citations for Human TSLP Antibody

Customer Reviews for Human TSLP Antibody (4)

3 out of 5
4 Customer Ratings
5 Stars
0%
4 Stars
50%
3 Stars
25%
2 Stars
0%
1 Stars
25%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human TSLP Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/09/2020
    We used this antibody in an in-house ELISA along with mAb (1398) and protein (1398-TS-010) to quantify TSLP in human serum and plasma. This combination could not detect TSLP samples but generated a good standard curve.
    Human TSLP Antibody AF1398
  • Human TSLP Antibody
    Name: avinash waghray
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 03/13/2019
    Did not work with and without citrate retrieval. IgG used as control and a different protein marking the same cells was seen.
    Human TSLP Antibody AF1398
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 20624642
    Species: Human
    Verified Customer | Posted 01/05/2015
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID 23335246
    Species: Human
    Verified Customer | Posted 01/05/2015

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