Detection of TSPAN1 in HT‑29 Human Cell Line by Flow Cytometry.
HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TSPAN1 Monoclonal Antibody (Catalog # MAB9186, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of TSPAN1 in HEK293 Human Cell Line Transfected with Human TSPAN1 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human TSPAN1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN1 Monoclonal Antibody (Catalog # MAB9186) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB007). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Tetraspanins (TSPAN family) are small integral proteins that contain four transmembrane domains with two different and well characterized extracellular domains. Tetraspanins are found on the plasma membrane and within various intracellular organelles and granules in nearly all cell and tissue types. They interact with one another and with other transmembrane molecules, including integrins, generating a ‘web' of interactions believed to control cell and membrane trafficking and compartmentalization. Tetraspanin-1 (TSPAN1), a 241 amino acid protein, interacts with the thiamine transporter-1 (THTR-1, SLC19A2) in human intestinal epithelial cells, linking TSPAN1 to the regulation of thiamine uptake. TSPAN1 has been found to be expressed in gastric, ovarian, colon, and other carcinomas.
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