Human TWEAK/TNFSF12 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TWEAK/TNFSF12 in ELISAs and Western blots. In sandwich immunoassays, less than 15% cross-reactivity with recombinant mouse TWEAK is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human TWEAK/TNFSF12
    Arg93-His249
    Accession # Q4ACW9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW)
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Human TWEAK/TNFSF12 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human TWEAK/TNFSF12 Antibody (Catalog # MAB1090)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human TWEAK/TNFSF12 Biotinylated Antibody (Catalog # BAF1090)
  • ELISA Standard
     
    Recombinant Human TWEAK/TNFSF12 Protein (Catalog # 1090-TW)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
TWEAK/TNFSF12 in human PBMCs. TWEAK/TNFSF12 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TWEAK/TNFSF12 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1090) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TWEAK/TNFSF12

TWEAK is a type II transmembrane protein belonging to the TNF superfamily (1). It contains a short cytoplasmic domain (amino acids (aa) 1-18), the transmembrane domain (aa 19-42) and an extracellular domain (aa 43-249). The extracellular domains of human and murine TWEAK share 89% aa sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage after Arg 93 suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells. At least two receptors that bind TWEAK have been identified (2-4). Death Receptor 3 (DR3), also known as TNFRSF12, Apo-3, LARD, WSL-1, or TRAMP, is a TNF receptor superfamily member that is expressed predominantly in tissues with high lymphocyte content (2). It has been suggested that induction of cell death by TWEAK-DR3 interaction involves the activation of NF-kappa B. In cells that lack DR3, alternate pathways of TWEAK-induced cell death mediated by receptors distinct from DR3 have been suggested (5, 6). TWEAK receptor (TWEAKR, alternatively known as FN14), is a novel TNF receptor superfamily member that also binds TWEAK (3, 4). It is a mitogen-inducible gene that is expressed in fibroblasts, hepetocellular carcinomas and endothelial cells. TWEAK-TWEAKR interaction has been shown to play a role in endothelial cell growth and migration. This effect of TWEAK is not mediated by an up-regulation of VEGF (7).

  • References:
    1. Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
    2. Marsters, S. et al. (1998) Current Biol. 8:525.
    3. Wiley, S.R. et al. (2001) Immunity, 15:837.
    4. Feng, S-L.Y. et al. (2000) Am J. Path. 156:1253.
    5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
    6. Schneider, P. et al. (1999) Eur. J. Immunol., 29:1785.
    7. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.
  • Long Name:
    TNF-like and Weak Inducer of Apoptosis
  • Entrez Gene IDs:
    8742 (Human); 21944 (Mouse); 360548 (Rat)
  • Alternate Names:
    APO3 ligand; APO3LMGC20669; DR3LGTWEAKAPO3/DR3 ligand; MGC129581; TNF-related weak inducer of apoptosis; TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TWEAK
Related Research Areas
Isotype Controls
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