Human TWEAK/TNFSF12 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1090
Human TWEAK/TNFSF12 Biotinylated Antibody in Immunocytochemistry
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Human TWEAK/TNFSF12 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TWEAK/TNFSF12 in ELISAs and Western blots. In sandwich immunoassays, less than 15% cross-reactivity with recombinant mouse TWEAK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TWEAK/TNFSF12
Arg93-His249
Accession # Q4ACW9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW)
Immunocytochemistry
5-15 µg/mL
See below

Human TWEAK/TNFSF12 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human TWEAK/TNFSF12 Antibody (Catalog # MAB1090)

Standard: Recombinant Human TWEAK/TNFSF12 Protein (Catalog # 1090-TW)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry TWEAK/TNFSF12 in human PBMCs. View Larger

TWEAK/TNFSF12 in human PBMCs. TWEAK/TNFSF12 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TWEAK/TNFSF12 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1090) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TWEAK/TNFSF12

TWEAK is a type II transmembrane protein belonging to the TNF superfamily (1). It contains a short cytoplasmic domain (amino acids (aa) 1-18), the transmembrane domain (aa 19-42) and an extracellular domain (aa 43-249). The extracellular domains of human and murine TWEAK share 89% aa sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage after Arg 93 suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells. At least two receptors that bind TWEAK have been identified (2-4). Death Receptor 3 (DR3), also known as TNFRSF12, Apo-3, LARD, WSL-1, or TRAMP, is a TNF receptor superfamily member that is expressed predominantly in tissues with high lymphocyte content (2). It has been suggested that induction of cell death by TWEAK-DR3 interaction involves the activation of NF-kappa B. In cells that lack DR3, alternate pathways of TWEAK-induced cell death mediated by receptors distinct from DR3 have been suggested (5, 6). TWEAK receptor (TWEAKR, alternatively known as FN14), is a novel TNF receptor superfamily member that also binds TWEAK (3, 4). It is a mitogen-inducible gene that is expressed in fibroblasts, hepetocellular carcinomas and endothelial cells. TWEAK-TWEAKR interaction has been shown to play a role in endothelial cell growth and migration. This effect of TWEAK is not mediated by an up-regulation of VEGF (7).

References
  1. Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
  2. Marsters, S. et al. (1998) Current Biol. 8:525.
  3. Wiley, S.R. et al. (2001) Immunity, 15:837.
  4. Feng, S-L.Y. et al. (2000) Am J. Path. 156:1253.
  5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
  6. Schneider, P. et al. (1999) Eur. J. Immunol., 29:1785.
  7. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.
Long Name
TNF-like and Weak Inducer of Apoptosis
Entrez Gene IDs
8742 (Human); 21944 (Mouse); 360548 (Rat)
Alternate Names
APO3 ligand; APO3LMGC20669; DR3LGTWEAKAPO3/DR3 ligand; MGC129581; TNF-related weak inducer of apoptosis; TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TWEAK

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