Human TWEAK/TNFSF12 Antibody

Catalog # Availability Size / Price Qty
AF1090
AF1090-SP
Human TWEAK/TNFSF12 Antibody in Neutralization
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human TWEAK/TNFSF12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TWEAK in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) BAFF, rhFas Ligand, rhGITR Ligand, rhOX40 Ligand, rhTRAIL and less than 1% cross-reactivity with rhAPRIL, rhLIGHT, rhTNF-alpha, rhVEGI, and rhTRANCE is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TWEAK
Arg93-His249
Accession # Q4ACW9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize TWEAK/TNFSF12-induced inhibition of proliferation in the HT‑29 human colon adenocarcinoma cell line. Yu, K. Y. et al. (1999) J. Biol. Chem. 274:13733; Harrop, J. A. et al. (1998) J. Biol. Chem. 273:27548The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 1  µg/mL Recombinant Human TWEAK/TNFSF12.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization TWEAK/TNFSF12 Inhibition of Cell Proliferation and Neutralization by Human TWEAK/TNFSF12 Antibody. View Larger

TWEAK/TNFSF12 Inhibition of Cell Proliferation and Neutralization by Human TWEAK/TNFSF12 Antibody. Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) inhibits proliferation in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human TWEAK/TNFSF12 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK/ TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090). The ND50is typically 2-8 µg/mL.

Immunocytochemistry TWEAK/TNFSF12 in MDA-MB-231 Human Cell Line. View Larger

TWEAK/TNFSF12 in MDA-MB-231 Human Cell Line. TWEAK/TNFSF12 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using 10 µg/mL Goat Anti-Human TWEAK/TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TWEAK/TNFSF12

TWEAK is a type II transmembrane protein belonging to the TNF superfamily (1). It contains a short cytoplasmic domain (amino acids (aa) 1-18), the transmembrane domain (aa 19-42) and an extracellular domain (aa 43-249). The extracellular domains of human and murine TWEAK share 89% aa sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage after Arg 93 suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells. At least two receptors that bind TWEAK have been identified (2-4). Death Receptor 3 (DR3), also known as TNFRSF12, Apo-3, LARD, WSL-1, or TRAMP, is a TNF receptor superfamily member that is expressed predominantly in tissues with high lymphocyte content (2). It has been suggested that induction of cell death by TWEAK-DR3 interaction involves the activation of NF-kappa B. In cells that lack DR3, alternate pathways of TWEAK-induced cell death mediated by receptors distinct from DR3 have been suggested (5, 6). TWEAK receptor (TWEAKR, alternatively known as FN14), is a novel TNF receptor superfamily member that also binds TWEAK (3, 4). It is a mitogen-inducible gene that is expressed in fibroblasts, hepetocellular carcinomas and endothelial cells. TWEAK-TWEAKR interaction has been shown to play a role in endothelial cell growth and migration. This effect of TWEAK is not mediated by an up-regulation of VEGF (7).

References
  1. Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
  2. Marsters, S. et al. (1998) Current Biol. 8:525.
  3. Wiley, S.R. et al. (2001) Immunity, 15:837.
  4. Feng, S-L.Y. et al. (2000) Am J. Path. 156:1253.
  5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
  6. Schneider, P. et al. (1999) Eur. J. Immunol., 29:1785.
  7. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.
Long Name
TNF-like and Weak Inducer of Apoptosis
Entrez Gene IDs
8742 (Human); 21944 (Mouse); 360548 (Rat)
Alternate Names
APO3 ligand; APO3LMGC20669; DR3LGTWEAKAPO3/DR3 ligand; MGC129581; TNF-related weak inducer of apoptosis; TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TWEAK

Product Datasheets

Citations for Human TWEAK/TNFSF12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.
    Authors: Stephan D, Sbai O, Wen J, Couraud P, Putterman C, Khrestchatisky M, Desplat-Jego S
    J Neuroinflammation, 2013;10(0):9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins.
    Authors: Inoue T, Tashiro Y, Takeuchi M
    Int. J. Oncol., 2011;38(5):1299-306.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
    Authors: Moreno JA, Dejouvencel T, Labreuche J, Smadja DM, Dussiot M, Martin-Ventura JL, Egido J, Gaussem P, Emmerich J, Michel JB, Blanco-Colio LM, Meilhac O
    Arterioscler. Thromb. Vasc. Biol., 2010;30(6):1253-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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Human TWEAK/TNFSF12 Antibody
By Anonymous on 11/08/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the detection in an ELISA with MAB1090 as the capture.