Human Tyk2 Antibody

Catalog # Availability Size / Price Qty
MAB3427
MAB3427-SP
Detection of Human Tyk2 by Western Blot.
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Human Tyk2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tyk2 in Western blots.
Source
Monoclonal Mouse IgG3 Clone # 307214
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Tyk2
Pro2-Leu51
Accession # P29597
Formulation
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Tyk2 by Western Blot. View Larger

Detection of Human Tyk2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Tyk2 Monoclonal Antibody (Catalog # MAB3427) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tyk2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyk2

Tyk2 belongs to the Janus family of intracellular tyrosine kinases that are activated by receptors for many cytokines. Activation of Tyk2 occurs via phosphorylation at two adjacent tyrosine residues, Y1054 and Y1055, within the kinase domain. Tyk2 is required for interferon beta -mediated tyrosine phosphorylation of STAT3 and interferon receptor 1. Mice lacking Tyk2 are susceptible to parasitic infection but resistant to autoimmune arthritis.

Long Name
Protein tyrosine kinase 2
Entrez Gene IDs
7297 (Human); 54721 (Mouse); 298706 (Rat)
Alternate Names
EC 2.7.10; EC 2.7.10.2; JTK1; non-receptor tyrosine-protein kinase TYK2; Tyk2; tyrosine kinase 2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Tyk2 Antibody

Average Rating: 3.5 (Based on 2 Reviews)

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Human Tyk2 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: A549 human lung carcinoma cell line,HCC827 human non-small cell lung cancer cell line Species: Human

Human Tyk2 Antibody
By Anonymous on 12/05/2017
Application: IHC Sample Tested: testes Species: human testes,Human

Week staining