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Human VASA Antibody

Catalog #: MAB2030
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2030
MAB2030-SP

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Detection of Human VASA by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VASA Antibody Summary

Species Reactivity
Human
Specificity
Detects human VASA in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 246904
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human VASA
Met1-Tyr145
Accession # Q9NQI0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human VASA antibody by Western Blot. View Larger

Detection of Human VASA by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VASA Monoclonal Antibody (Catalog # MAB2030) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VASA

VASA, also known as DDX4, is a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is a highly specific marker of germ cells.

Long Name
DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41
Entrez Gene IDs
54514 (Human); 13206 (Mouse); 310090 (Rat)
Alternate Names
DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; EC 3.6.1; EC 3.6.4.13; MGC111074; probable ATP-dependent RNA helicase DDX4; Vasa homolog; VASA; VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

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Citations for Human VASA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells
    Authors: Christopher Noyes, Shunsuke Kitajima, Fengkai Li, Yusuke Suita, Saradha Miriyala, Shakson Isaac et al.
    Communications Biology
  2. The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells
    Authors: Christopher Noyes, Shunsuke Kitajima, Fengkai Li, Yusuke Suita, Saradha Miriyala, Shakson Isaac et al.
    Communications Biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry

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