Best Seller

Human VEGF Quantikine ELISA Kit

R&D Systems | Catalog # DVE00

R&D Systems
100% Guarantee

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)

Sensitivity

5 pg/mL (Cell Culture Supernates)
9 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates)
31.3-2000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
View all VEGF ELISA Kits »

Product Summary for Human VEGF Quantikine ELISA Kit

The Quantikine Human VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF165 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human VEGF165 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human VEGF and recombinant human VEGF121 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human VEGF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human VEGF. This assay also recognizes recombinant human VEGF165b.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity observed with 1 or more species tested.

Interference

Interference observed with 1 or more available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human VEGF in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean of detectable (pg/mL)% DetectableRange (pg/mL)
Serum (n=37)22010062-707
EDTA plasma (n=37)6124ND-115
Heparin plasma (n=37)4122ND-55
Citrate plasma (n=37)___0ND
ND=Non-Detectable

Cell Culture Supernates: 
Human peripheral blood mononuclear cells (10 x 106 cells/mL) were cultured in RPMI supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed, assayed for levels of human VEGF, and measured 87.1 pg/mL and 1041 pg/mL, respectively.

PC-3 human prostate cancer cells were cultured in RPMI supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 60 nM of PMA for 24 hours. Aliquots of the cell culture supernates were removed, assayed for levels of human VEGF, and measured 3373 pg/mL and 3652 pg/mL, respectively.

JEG-3 human epithelial choriocarcinoma cells were cultured in MEM + NEAA supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 100 ng/mL of LPS for 1 day. Aliquots of the cell culture supernates were removed, assayed for levels of human VEGF, and measured 1538 pg/mL and 1938 pg/mL, respectively. 

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 29.1 123 531 32.8 128 495
Standard Deviation 1.9 5.0 18.4 2.8 6.4 33.0
CV% 6.5 4.1 3.5 8.5 5.0 6.7

Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 53.7 235 910 64.5 250 1003
Standard Deviation 3.6 10.6 46.2 5.7 17.4 61.7
CV% 6.7 4.5 5.1 8.8 7.0 6.2

Recovery for Human VEGF Quantikine ELISA Kit

The recovery of human VEGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 102 95-111
Citrate Plasma (n=5) 100 88-113
EDTA Plasma (n=5) 97 82-113
Heparin Plasma (n=5) 93 82-102
Serum (n=5) 102 92-115

Linearity

To assess linearity of the assay, samples were spiked with high concentrations of human VEGF and diluted with the appropriate calibrator diluent to produce samples with values within the dynamic range of the assay.

Human VEGF ELISA Linearity

Scientific Data Images for Human VEGF Quantikine ELISA Kit

Human VEGF ELISA Cell Culture Supernate Standard Curve

Human VEGF ELISA Cell Culture Supernate Standard Curve

Human VEGF ELISA Serum/Plasma Standard Curve

Human VEGF ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and the formation of antiparallel disulfide-linked dimers (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle (5, 6), hepatocytes (7), osteoblasts (8), neutrophils (9), macrophages (10), keratinocytes (11), brown adipose tissue (12), CD34+ stem cells (13), endothelial cells (14), fibroblasts, and vascular smooth muscle cells (15). VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M, and TNF-alpha (3, 4, 9, 16). VEGF isoforms are differentially expressed during development and in the adult (3). 
VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR), and induce their homodimerization and autophosphorylation (3, 4, 7, 17, 18). These receptors have seven extracellular immunoglobulin-like domains and an intracellular split tyrosine kinase domain. They are expressed on vascular endothelial cells and a range of non-endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 also binds the semaphorin receptor, neuropilin-1, which promotes complex formation with VEGF R2 (19). 
VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells (3, 4), thus regulating blood vessel density and size, but playing no role in determining vascular patterns. VEGF promotes bone formation through osteoblast and chondroblast recruitment and is also a monocyte chemoattractant (20-22). After birth, VEGF maintains endothelial cell integrity and is a potent mitogen for micro- and macro-vascular endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). In diseased tissues, VEGF promotes vascular permeability. It is thus thought to contribute to tumor metastasis by promoting both extravasation and tumor angiogenesis (23, 24). Various strategies have been employed therapeutically to antagonize VEGF-mediated tumor angiogenesis (25). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (26).

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

Additional VEGF Products

Product Documents for Human VEGF Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VEGF Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human VEGF Quantikine ELISA Kit

Customer Reviews for Human VEGF Quantikine ELISA Kit (29)

4.9 out of 5
29 Customer Ratings
5 Stars
86%
4 Stars
14%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human VEGF Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 of 29 reviews Showing All
Filter By:
Clear Filters X
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 11/10/2023
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum and Lung cancer tissue
    Verified Customer | Posted 10/31/2023
    We ordered a large batch of reagent kits together to study the interaction between blood vessel formation and the microenvironment. The results are evident, and the reagent kit testing is very sensitive.
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Andres Ocampo
    Sample Tested: Human fibroblast
    Verified Customer | Posted 10/11/2022
    Human VEGF was measured in human HUVEC Cells after stimulation with IFN-g and IL-1b. An increased production was significantly obtained, compared to unstimulated cells.
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Mike Ehrhardt
    Sample Tested: Serum-free Cell Culture Supernates
    Verified Customer | Posted 11/24/2020
    This ELISA kit performed as expected. Very low % CV's
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: HeLa cells
    Verified Customer | Posted 08/26/2020
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Media
    Verified Customer | Posted 01/07/2020
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 12/09/2019
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: in vitro cell culture
    Verified Customer | Posted 10/19/2019
  • Human VEGF Quantikine ELISA Kit
    Name: Jenna Nguyen
    Sample Tested: 4T1.2 mouse mammary carcinoma cells
    Verified Customer | Posted 10/17/2019
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Bone Extracts
    Verified Customer | Posted 10/10/2019
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: in vitro cell lysis
    Verified Customer | Posted 08/13/2019
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Verified Customer | Posted 04/30/2019
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Brain Microvascular Endothelial Cells
    Verified Customer | Posted 03/21/2019
  • Human VEGF Quantikine ELISA Kit
    Name: Bo Yu
    Sample Tested: Cell Culture Media
    Verified Customer | Posted 11/09/2018
    Just follow the protocol. Very easy.
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Jeff Pimentel
    Sample Tested: Serum
    Verified Customer | Posted 06/12/2018
  • Human VEGF Quantikine ELISA Kit
    Name: Danlee Enzler
    Sample Tested: B16-F10 mouse melanoma cell line
    Verified Customer | Posted 04/18/2018
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 04/05/2018
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Leslie Priddy
    Sample Tested: Serum
    Verified Customer | Posted 03/30/2018
  • Human VEGF Quantikine ELISA Kit
    Name: Gongxian Liao
    Sample Tested: In vitro biochemical assay
    Verified Customer | Posted 03/22/2018
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 01/12/2018
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Lina Guo
    Sample Tested: Human cell conditioned medium, A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell line
    Verified Customer | Posted 10/06/2017
    I have used this kit for a very large part of my PhD project on >20 cell lines. It worked every time, giving reproducible and reliable results with great sensibility. Highly recommend this.
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell Culture Media
    Verified Customer | Posted 09/27/2017
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Human skeletal muscle
    Verified Customer | Posted 04/21/2017
    Followed instructions for serum/plasma. Ran samples full strength
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 01/23/2017
    This VEGF ELISA performs very well and very reproducible. We have used different lot#s, all perform very equally. Well calibrated Standard Protein si provided, curve is almost identical to reference Standard (NIBSC). We usually extend the Standard curve by an additional low calibrator Point (additional 1:2 Dilution of S7), because the Assay still performes well at the low Assay range. Human EDTA-Plasma samples are usually quantifiable and above the detection limit. In general human blood samples matrix should be selected carefully to avoid effects from VEGF release during coagulation.
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Hematopoietic Stem Cells
    Verified Customer | Posted 12/14/2016
    In our lab, we developed serum-free culture media to expand human hematopoietic stem cells and human endothelial progenitor cells in vitro. We also tried to cocluture both cells. After culture, Human VEGF Quantikine ELISA Kit were used to check the cytokine, VEGF secretion.
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: iPSC-derived neural cells
    Verified Customer | Posted 12/14/2016
    I used this ELISA Kit to measure VEGF secretion in iPSC-derived astrocytes, brain microvascular endothelial cells and neurons following exposure to hypoxic/ischemic injury in vitro. The kit was easy to use, well detailed and provided enough reagents to successfully run the assay.
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Adipose tissue
    Verified Customer | Posted 06/20/2016
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Sharon Hechter
    Sample Tested: IMR-32 human neuroblastoma cell line
    Verified Customer | Posted 06/17/2016
    Human VEGF Quantikine ELISA Kit DVE00
  • Human VEGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Bone Putty
    Verified Customer | Posted 05/09/2016
    Human VEGF Quantikine ELISA Kit DVE00

There are no reviews that match your criteria.

Showing  1 - 5 of 29 reviews Showing All

Protocols

View specific protocols for Human VEGF Quantikine ELISA Kit (DVE00):

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

  1. Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2. Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
  3. For Serum & Plasma Samples: Add 100 µL of Assay Diluent to each well.
    For Cell Culture Supernate Samples: Add 50 µL Assay Diluent to each well.

    4. Add Assay Diluent


    assay procedure
  4. For Serum & Plasma Samples: Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples: Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5. Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    6. Add Standard, Control, or Sample


    assay procedure
  6. Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7. Aspirate and wash 3 times.

    8. 200 µL Conjugate


    assay procedure
  8. Add 200 µL Substrate Solution to each well.
  9. For Serum & Plasma Samples: Incubate at room temperature for 25 minutes. PROTECT FROM LIGHT. For Cell Culture Supernate Samples: Incubate at room temperature for 20 minutes.
    PROTECT FROM LIGHT.

    10. 200 µL Substrate Solution


    assay procedure substrate solution
  10. For Serum & Plasma Samples: Incubate at room temperature for 25 minutes. PROTECT FROM LIGHT. For Cell Culture Supernate Samples: Incubate at room temperature for 20 minutes.
    PROTECT FROM LIGHT.

    11. 50 µL Stop Solution


    assay procedure stop solution

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human VEGF Quantikine ELISA Kit

Showing  1 - 5 of 6 FAQs Showing All

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect human VEGF121?

    A: Yes, the kit detects VEGF121.  In specificity testing, 100% cross-reactivity was observed with recombinant human VEGF121. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect mouse VEGF?

    A: No, the kit does not detect mouse VEGF. Specificity testing with recombinant mouse VEGF 120 and VEGF 164 showed no cross-reactivity. For the detection of mouse VEGF, please refer to the Mouse VEGF Quantikine® ELISA kit (Catalog # MMV00).

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect porcine samples?

    A: Our lab has not tested porcine samples in the kit, however many customers have published use of the kit with porcine samples.  Links to these references can be found in the citations tab by selecting for Porcine in the Species filter.

  • Q: How long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGF using the DVE00 Human VEGF Quantikine® ELISA Kit?

    A: R&D Systems does not evaluate sample stability. Variations in sample collection, processing, and storage may affect stability of samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. Avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

  • Q: Is the heterodimer VEGF165/PlGF detected by DVE00? 

    A: VEGF165/PlGF is not detected by this kit. Recombinant human VEGF165/PlGF was prepared at 50 ng/mL and assayed for cross-reactivity, with negative results.

  • Q: Is there a protocol for using cell lysates with the Human VEGF Quantikine® ELISA Kit (Catalog # DVE00)?

    A: R&D Systems does not have a validated cell lysate protocol available for this kit.  However, many customers have published use of the kit with cell lysates.  Links to these references can be found in the citations tab.  Generally, the use of detergents in the lysis buffer should be avoided when preparing cell samples.  A better option is to lyse the cells via freeze/thaw cycles or by sonication. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect human VEGF121?

    A: Yes, the kit detects VEGF121.  In specificity testing, 100% cross-reactivity was observed with recombinant human VEGF121. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect mouse VEGF?

    A: No, the kit does not detect mouse VEGF. Specificity testing with recombinant mouse VEGF 120 and VEGF 164 showed no cross-reactivity. For the detection of mouse VEGF, please refer to the Mouse VEGF Quantikine® ELISA kit (Catalog # MMV00).

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect porcine samples?

    A: Our lab has not tested porcine samples in the kit, however many customers have published use of the kit with porcine samples.  Links to these references can be found in the citations tab by selecting for Porcine in the Species filter.

  • Q: How long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGF using the DVE00 Human VEGF Quantikine® ELISA Kit?

    A: R&D Systems does not evaluate sample stability. Variations in sample collection, processing, and storage may affect stability of samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. Avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

  • Q: Is the heterodimer VEGF165/PlGF detected by DVE00? 

    A: VEGF165/PlGF is not detected by this kit. Recombinant human VEGF165/PlGF was prepared at 50 ng/mL and assayed for cross-reactivity, with negative results.

  • Q: Is there a protocol for using cell lysates with the Human VEGF Quantikine® ELISA Kit (Catalog # DVE00)?

    A: R&D Systems does not have a validated cell lysate protocol available for this kit.  However, many customers have published use of the kit with cell lysates.  Links to these references can be found in the citations tab.  Generally, the use of detergents in the lysis buffer should be avoided when preparing cell samples.  A better option is to lyse the cells via freeze/thaw cycles or by sonication. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect human VEGF121?

    A: Yes, the kit detects VEGF121.  In specificity testing, 100% cross-reactivity was observed with recombinant human VEGF121. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect mouse VEGF?

    A: No, the kit does not detect mouse VEGF. Specificity testing with recombinant mouse VEGF 120 and VEGF 164 showed no cross-reactivity. For the detection of mouse VEGF, please refer to the Mouse VEGF Quantikine® ELISA kit (Catalog # MMV00).

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect porcine samples?

    A: Our lab has not tested porcine samples in the kit, however many customers have published use of the kit with porcine samples.  Links to these references can be found in the citations tab by selecting for Porcine in the Species filter.

  • Q: How long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGF using the DVE00 Human VEGF Quantikine® ELISA Kit?

    A: R&D Systems does not evaluate sample stability. Variations in sample collection, processing, and storage may affect stability of samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. Avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

  • Q: Is the heterodimer VEGF165/PlGF detected by DVE00? 

    A: VEGF165/PlGF is not detected by this kit. Recombinant human VEGF165/PlGF was prepared at 50 ng/mL and assayed for cross-reactivity, with negative results.

  • Q: Is there a protocol for using cell lysates with the Human VEGF Quantikine® ELISA Kit (Catalog # DVE00)?

    A: R&D Systems does not have a validated cell lysate protocol available for this kit.  However, many customers have published use of the kit with cell lysates.  Links to these references can be found in the citations tab.  Generally, the use of detergents in the lysis buffer should be avoided when preparing cell samples.  A better option is to lyse the cells via freeze/thaw cycles or by sonication. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect human VEGF121?

    A: Yes, the kit detects VEGF121.  In specificity testing, 100% cross-reactivity was observed with recombinant human VEGF121. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect mouse VEGF?

    A: No, the kit does not detect mouse VEGF. Specificity testing with recombinant mouse VEGF 120 and VEGF 164 showed no cross-reactivity. For the detection of mouse VEGF, please refer to the Mouse VEGF Quantikine® ELISA kit (Catalog # MMV00).

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect porcine samples?

    A: Our lab has not tested porcine samples in the kit, however many customers have published use of the kit with porcine samples.  Links to these references can be found in the citations tab by selecting for Porcine in the Species filter.

  • Q: How long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGF using the DVE00 Human VEGF Quantikine® ELISA Kit?

    A: R&D Systems does not evaluate sample stability. Variations in sample collection, processing, and storage may affect stability of samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. Avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

  • Q: Is the heterodimer VEGF165/PlGF detected by DVE00? 

    A: VEGF165/PlGF is not detected by this kit. Recombinant human VEGF165/PlGF was prepared at 50 ng/mL and assayed for cross-reactivity, with negative results.

  • Q: Is there a protocol for using cell lysates with the Human VEGF Quantikine® ELISA Kit (Catalog # DVE00)?

    A: R&D Systems does not have a validated cell lysate protocol available for this kit.  However, many customers have published use of the kit with cell lysates.  Links to these references can be found in the citations tab.  Generally, the use of detergents in the lysis buffer should be avoided when preparing cell samples.  A better option is to lyse the cells via freeze/thaw cycles or by sonication. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect human VEGF121?

    A: Yes, the kit detects VEGF121.  In specificity testing, 100% cross-reactivity was observed with recombinant human VEGF121. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect mouse VEGF?

    A: No, the kit does not detect mouse VEGF. Specificity testing with recombinant mouse VEGF 120 and VEGF 164 showed no cross-reactivity. For the detection of mouse VEGF, please refer to the Mouse VEGF Quantikine® ELISA kit (Catalog # MMV00).

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect porcine samples?

    A: Our lab has not tested porcine samples in the kit, however many customers have published use of the kit with porcine samples.  Links to these references can be found in the citations tab by selecting for Porcine in the Species filter.

  • Q: How long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGF using the DVE00 Human VEGF Quantikine® ELISA Kit?

    A: R&D Systems does not evaluate sample stability. Variations in sample collection, processing, and storage may affect stability of samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. Avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

  • Q: Is the heterodimer VEGF165/PlGF detected by DVE00? 

    A: VEGF165/PlGF is not detected by this kit. Recombinant human VEGF165/PlGF was prepared at 50 ng/mL and assayed for cross-reactivity, with negative results.

  • Q: Is there a protocol for using cell lysates with the Human VEGF Quantikine® ELISA Kit (Catalog # DVE00)?

    A: R&D Systems does not have a validated cell lysate protocol available for this kit.  However, many customers have published use of the kit with cell lysates.  Links to these references can be found in the citations tab.  Generally, the use of detergents in the lysis buffer should be avoided when preparing cell samples.  A better option is to lyse the cells via freeze/thaw cycles or by sonication. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect human VEGF121?

    A: Yes, the kit detects VEGF121.  In specificity testing, 100% cross-reactivity was observed with recombinant human VEGF121. 

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect mouse VEGF?

    A: No, the kit does not detect mouse VEGF. Specificity testing with recombinant mouse VEGF 120 and VEGF 164 showed no cross-reactivity. For the detection of mouse VEGF, please refer to the Mouse VEGF Quantikine® ELISA kit (Catalog # MMV00).

  • Q: Does the Human VEGF Quantikine® ELISA kit (Catalog # DVE00) detect porcine samples?

    A: Our lab has not tested porcine samples in the kit, however many customers have published use of the kit with porcine samples.  Links to these references can be found in the citations tab by selecting for Porcine in the Species filter.

  • Q: How long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGF using the DVE00 Human VEGF Quantikine® ELISA Kit?

    A: R&D Systems does not evaluate sample stability. Variations in sample collection, processing, and storage may affect stability of samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. Avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

  • Q: Is the heterodimer VEGF165/PlGF detected by DVE00? 

    A: VEGF165/PlGF is not detected by this kit. Recombinant human VEGF165/PlGF was prepared at 50 ng/mL and assayed for cross-reactivity, with negative results.

  • Q: Is there a protocol for using cell lysates with the Human VEGF Quantikine® ELISA Kit (Catalog # DVE00)?

    A: R&D Systems does not have a validated cell lysate protocol available for this kit.  However, many customers have published use of the kit with cell lysates.  Links to these references can be found in the citations tab.  Generally, the use of detergents in the lysis buffer should be avoided when preparing cell samples.  A better option is to lyse the cells via freeze/thaw cycles or by sonication. 

Showing  1 - 5 of 6 FAQs Showing All
View all FAQs for ELISA Kits