VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Met1-Gly245
Accession # Q5DX21
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human VSIG3 Antibody
Detection of VSIG3 in HEK293 human cell line transfected with human VSIG3 by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) Human VSIG3 or (B) irrelevant protein, and EGFP, was stained with Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB92292) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.
Applications for Human VSIG3 Antibody
CyTOF-ready
Flow Cytometry
Sample: HEK293 human cell line transfected with human VSIG3
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: VSIG3
Long Name
Alternate Names
Gene Symbol
UniProt
Additional VSIG3 Products
Product Documents for Human VSIG3 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human VSIG3 Antibody
For research use only
Related Research Areas
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Protocols
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