Human vWF-A2 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY2764-05
Ancillary Products Available
Human vWF-A2 ELISA Standard Curve
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Human vWF-A2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant vWF-A2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human vWF-A2 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: vWF-A2

Human vWF is a serum glycoprotein that mediates platelet adhesion to injured vascular endothelium. vWF function is regulated by ADAMTS13 mediated proteolysis within the A2 domain. Mutations of this protease cause thrombotic thrombocytopenic purpura.

Long Name:
von Willebrand Factor A2 Domain
Entrez Gene IDs:
7450 (Human); 22371 (Mouse); 116669 (Rat)
Alternate Names:
F8VWF; VWD; VWF von Willebrand factor; VWF; vWFA2; vWF-A2

Citations for Human vWF-A2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Levels and prognostic impact of circulating markers of inflammation, endothelial activation and extracellular matrix remodelling in patients with lung cancer and chronic obstructive pulmonary disease
    Authors: J Berg, AR Halvorsen, MB Bengtson, KA Taskén, GM Mælandsmo, A Yndestad, B Halvorsen, OT Brustugun, P Aukrust, T Ueland, Å Helland
    BMC Cancer, 2018;18(1):739.
    Species: Human
    Sample Types: Serum
  2. Short-term effects of particle size fractions on circulating biomarkers of inflammation in a panel of elderly subjects and healthy young adults
    Authors: MS Hassanvand, K Naddafi, H Kashani, S Faridi, N Kunzli, R Nabizadeh, F Momeniha, A Gholampour, M Arhami, A Zare, Z Pourpak, M Hoseini, M Yunesian
    Environ. Pollut, 2017;223(0):695-704.
    Species: Human
    Sample Types: Plasma
  3. Impact of Unsaturated Fatty Acids on Cytokine-Driven Endothelial Cell Dysfunction
    Authors: S Trommer, A Leimert, M Bucher, J Schumann
    Int J Mol Sci, 2017;18(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Follicular fluid cytokine composition and oocyte quality of polycystic ovary syndrome patients with metabolic syndrome undergoing in vitro fertilization
    Authors: Z Niu, Y Ye, L Xia, Y Feng, A Zhang
    Cytokine, 2017;91(0):180-186.
    Species: Human
    Sample Types: Follicular Fluid

FAQs

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