Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 336918
View all WASP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human WASP
Lys230-Gln310 (predicted)
Accession # P42768

Specificity

Detects human WASP in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human WASP Antibody

Detection of Human WASP antibody by Western Blot.

Detection of Human WASP by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2.5 µg/mL of Mouse Anti-Human WASP Monoclonal Antibody (Catalog # MAB30701) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for WASP at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human WASP Antibody

Application
Recommended Usage

Western Blot

2.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: WASP

WASP (Wiskott-Aldrich Syndrome Protein) is a 54 kDa protein belonging to the WASP/SCAR/WAVE family of proteins involved in actin polymerization. It is a highly conserved protein and is expressed selectively in hematopoietic stem cell-derived lineages. WASP contains multiple domains with unique functions. Mutations in WASP are associated with diseases of the hematopoietic/ immunologic system.

Long Name

Wiskott-Aldrich Syndrome Protein

Alternate Names

Thrombocytopenia 1, WAS

Entrez Gene IDs

7454 (Human); 22376 (Mouse)

Gene Symbol

WAS

UniProt

P42768

Additional WASP Products

Product Documents for Human WASP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human WASP Antibody

For research use only

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FAQs

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