Human ZNF24 Antibody

Catalog # Availability Size / Price Qty
AF3637
AF3637-SP
ZNF24 in HepG2 Human Cell Line.
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Product Details
Citations (1)
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Human ZNF24 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ZNF24 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ZNF24
Ser2-Val368
Accession # NP_008896
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ZNF24
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry ZNF24 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

ZNF24 in HepG2 Human Cell Line. ZNF24 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human ZNF24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3637) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZNF24

Human ZNF24, also known as ZNF191, is a 42 kDa transcription regulator that belongs to the Kruppel-like zinc finger gene family. It contains an N-terminal SCAN box domain and four C-terminal tandem zinc finger motifs. ZNF24 is ubiquitously expressed. A second splice isoform that contains a 4 residue substitution for aa 191‑368 of full-length ZNF24, has been reported. Human and mouse ZNF24 share 95% amino acid sequence homology.

Long Name
Zinc Finger Protein 24
Entrez Gene IDs
7572 (Human); 331360 (Mouse)
Alternate Names
FLJ32104; KOX17; RSG-A; Zfp191; zinc finger protein 488; ZNF24; ZSCAN3

Product Datasheets

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Citation for Human ZNF24 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Transcriptional repression of VEGF by ZNF24: mechanistic studies and vascular consequences in vivo.
    Authors: Jia, Di, Hasso, Sean M, Chan, Joanne, Filingeri, Domenic, D'Amore, Patricia, Rice, Lori, Pampo, Christin, Siemann, Dietmar, Zurakowski, David, Rodig, Scott J, Moses, Marsha A
    Blood, 2013;121(4):707-15.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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