Human ZNF24 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ZNF24 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human ZNF24
    Ser2-Val368
    Accession # NP_008896
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human ZNF24
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
ZNF24 in HepG2 Human Cell Line. ZNF24 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human ZNF24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3637) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZNF24
Human ZNF24, also known as ZNF191, is a 42 kDa transcription regulator that belongs to the Kruppel-like zinc finger gene family. It contains an N-terminal SCAN box domain and four C-terminal tandem zinc finger motifs. ZNF24 is ubiquitously expressed. A second splice isoform that contains a 4 residue substitution for aa 191‑368 of full-length ZNF24, has been reported. Human and mouse ZNF24 share 95% amino acid sequence homology.
  • Long Name:
    Zinc Finger Protein 24
  • Entrez Gene IDs:
    7572 (Human); 331360 (Mouse)
  • Alternate Names:
    FLJ32104; KOX17; RSG-A; Zfp191; zinc finger protein 488; ZNF24; ZSCAN3
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Transcriptional repression of VEGF by ZNF24: mechanistic studies and vascular consequences in vivo.
    Authors: Jia, Di, Hasso, Sean M, Chan, Joanne, Filingeri, Domenic, D'Amore, Patricia, Rice, Lori, Pampo, Christin, Siemann, Dietmar, Zurakowski, David, Rodig, Scott J, Moses, Marsha A
    Blood, 2013;121(4):707-15.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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