IGF-I/IGF-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16929

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Predicted:

Bovine (97%), Canine (98%), Guinea Pig (100%), Porcine (96%), Rat (94%), Rhesus Macaque (100%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IGF-I/IGF-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGF-I/IGF-1. The exact sequence is proprietary.

Reactivity Notes

Armadillo (100%), Xenopus laevis (80%), Rabbit (82%), Chicken (85%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IGF-I/IGF-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929]

Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929]

Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
IGF-I/IGF-1 Antibody - BSA Free

Western Blot: IGF-I/IGF-1 Antibody [NBP2-16929] -

Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (NBP2-16929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
IGF-I/IGF-1 Antibody - BSA Free

Western Blot: IGF-I/IGF-1 Antibody [NBP2-16929] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (NBP2-16929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
IGF-I/IGF-1 Antibody - BSA Free

Western Blot: IGF-I/IGF-1 Antibody [NBP2-16929] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (NBP2-16929) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
IGF-I/IGF-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody [NBP2-16929] -

IGF1 antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGF1 protein stained by IGF1 antibody (NBP2-16929) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for IGF-I/IGF-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-16929 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IGF-I/IGF-1

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

Additional IGF-I/IGF-1 Products

Product Documents for IGF-I/IGF-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IGF-I/IGF-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for IGF-I/IGF-1 Antibody - BSA Free

Customer Reviews for IGF-I/IGF-1 Antibody - BSA Free (1)

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  • Name: Andrew Cosgrove
    Application: Western Blot
    Sample Tested: Mouse
    Species: Mouse
    Verified Customer | Posted 12/16/2013
    NBP2-16929 in Mice
    IGF-I/IGF-1 Antibody - BSA Free NBP2-16929

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Protocols

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FAQs

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