IKK beta Antibody - BSA Free

Name: IKK beta Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-32258
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-32258

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Citations (3)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human IKK beta. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IKK beta Antibody - BSA Free

Western Blot: IKK beta Antibody [NBP1-32258]

Western Blot: IKK beta Antibody [NBP1-32258] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:500.

Immunocytochemistry/ Immunofluorescence: IKK beta Antibody [NBP1-32258]

Immunocytochemistry/Immunofluorescence: IKK beta Antibody [NBP1-32258] - analysis of A431, using IKK beta antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: IKK beta Antibody [NBP1-32258]

Immunohistochemistry-Paraffin: IKK beta Antibody [NBP1-32258] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Western Blot: IKK beta Antibody [NBP1-32258]

Western Blot: IKK beta Antibody [NBP1-32258] - IKK beta antibody detects IKK beta protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of DDDDK-human IKBKB-transfected 293T cells. 7.5 % SDS-PAGE. IKK beta antibody dilution: 1:5000

Western Blot: IKK beta Antibody [NBP1-32258]

Western Blot: IKK beta Antibody [NBP1-32258] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:1000.

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Applications for IKK beta Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IKK beta

IKK-beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta (3). Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response (4). Additionally, IKK-beta activity can also be regulated by MEKK-1 (5).

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Gene Symbol

IKBKB

Additional IKK beta Products

Product Documents for IKK beta Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for IKK beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.