IL-21R Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76739
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a synthetic peptide corresponding to amino acids 97 to 111 of human IL-21 receptor precursor. The immunogen is located within amino acids 80 - 130 of IL-21 Receptor.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IL-21R Antibody - BSA Free
Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739]
IL-21R-Antibody-Immunohistochemistry-NBP1-76739-img0006.jpgWestern Blot: IL-21R AntibodyBSA Free [NBP1-76739]
Western Blot: IL-21R Antibody [NBP1-76739] - Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 ug /ml.Immunohistochemistry-Paraffin: IL-21R Antibody - BSA Free [NBP1-76739]
Immunohistochemistry-Paraffin: IL-21R Antibody [NBP1-76739] - Rat lung with IL-21 receptor antibody at 10 ug/ml.Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] -
Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] - Treatment of avian xenografts with IL-21 upregulates Blimp-1 expression. (A) The three-dimensional growth of BxPC-3 cells transplanted on the CAM of fertilized chicken eggs & resected tumors (three replicates) from vehicle control & IL-21 were photographed at day 17 of embryonic development. Upper row for vehicle control; lower row for IL-21-treated (150 ng/mL) tumors. (B) HE & IL-21R (brown) IHC staining of vehicle control & IL-21-treated (150 ng/mL) resected xenograft tumors. (C) Comparison & quantification of Blimp-1 (nuclei: red) expression of vehicle control & IL-21-treated (150 ng/mL) xenograft tumors. **** P < 0.0001. Unpaired t-test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31540511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] -
Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] - IL-21R, IL-21, & Blimp-1 expression in human PDAC & healthy pancreas tissue samples. (A) Infiltration of IL-21+ immune cells (red) in PDAC. Black arrow: IL-21+ cells. (B) Infiltration of IL-21+ Th17 cells in PDAC: IL-21+ (red) & RORC+ (brown) cells. Black arrowhead: PDAC tumor cells, black arrow: IL-21+/RORC+ immune cells. (C) Examples of negative, weak & high expression of IL-21R (brown) & Blimp-1 (red) in human PDAC tissue. Black arrows: tumor cells. (D) Examples of IL-21R & Blimp-1 expression in healthy pancreas tissue. (E) IL-21R expression in human PDAC varied among the patients. (F) Association of IL-21R & Blimp-1 expression in human PDAC. * P < 0.05 & **** P < 0.0001. Unpaired t-test. (G) Co-expression of IL-21R (brown, membrane) & Blimp-1 (red, nucleus). Black arrowhead: IL-21R+/Blimp-1+, white arrow head: IL-21R+/Blimp-1−. Black bar: 20 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31540511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: IL-21R Antibody - BSA Free [NBP1-76739] -
Western Blot: IL-21R Antibody - BSA Free [NBP1-76739] - Western blot analysis of IL-21R in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 u/ml.Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] -
Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] - Immunofluorescence of IL21R in A431 cells with IL 21 Receptor antibody at 20 u/mL.Green: IL-21 Receptor Antibody
Blue: DAPI staining
Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] -
Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] - Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 u/mL.Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] -
IL-21R expression in human PDAC cell lines. Untreated human PDAC cell lines AsPC-1, BxPC-3, and Panc-1 were tested for IL-21R expression in (A) immunofluorescence staining (left: IL-21R+ cells, right: Alexa488 goat anti-rabbit IgG only; nuclei: DAPI (blue), IL-21R: Alexa488 (green)) and (B) western blot. White bar: 20 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31540511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for IL-21R Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1-2 ug/ml
Application Notes
An approximately 60 kDa band can be detected in Western Blot. Use in ICC/IF was reported in scientific literature (PMID: 31540511).
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-21R
Long Name
Interleukin 21 Receptor
Alternate Names
CD360, IL-21 R, IL21R, NILR
Gene Symbol
IL21R
UniProt
Additional IL-21R Products
Product Documents for IL-21R Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-21R Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for IL-21R Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars