IL-21R Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76739

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to amino acids 97 to 111 of human IL-21 receptor precursor. The immunogen is located within amino acids 80 - 130 of IL-21 Receptor.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-21R Antibody - BSA Free

Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739]

Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739]

IL-21R-Antibody-Immunohistochemistry-NBP1-76739-img0006.jpg
Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739]

Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739]

IL-21R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76739-img0007.jpg
Western Blot: IL-21R AntibodyBSA Free [NBP1-76739]

Western Blot: IL-21R AntibodyBSA Free [NBP1-76739]

IL-21R-Antibody-Western-Blot-NBP1-76739-img0008.jpg
Western Blot: IL-21R AntibodyBSA Free [NBP1-76739]

Western Blot: IL-21R AntibodyBSA Free [NBP1-76739]

Western Blot: IL-21R Antibody [NBP1-76739] - Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 ug /ml.
Immunohistochemistry-Paraffin: IL-21R Antibody - BSA Free [NBP1-76739]

Immunohistochemistry-Paraffin: IL-21R Antibody - BSA Free [NBP1-76739]

Immunohistochemistry-Paraffin: IL-21R Antibody [NBP1-76739] - Rat lung with IL-21 receptor antibody at 10 ug/ml.
IL-21R Antibody - BSA Free

Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] -

Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] - Treatment of avian xenografts with IL-21 upregulates Blimp-1 expression. (A) The three-dimensional growth of BxPC-3 cells transplanted on the CAM of fertilized chicken eggs & resected tumors (three replicates) from vehicle control & IL-21 were photographed at day 17 of embryonic development. Upper row for vehicle control; lower row for IL-21-treated (150 ng/mL) tumors. (B) HE & IL-21R (brown) IHC staining of vehicle control & IL-21-treated (150 ng/mL) resected xenograft tumors. (C) Comparison & quantification of Blimp-1 (nuclei: red) expression of vehicle control & IL-21-treated (150 ng/mL) xenograft tumors. **** P < 0.0001. Unpaired t-test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31540511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
IL-21R Antibody - BSA Free

Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] -

Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739] - IL-21R, IL-21, & Blimp-1 expression in human PDAC & healthy pancreas tissue samples. (A) Infiltration of IL-21+ immune cells (red) in PDAC. Black arrow: IL-21+ cells. (B) Infiltration of IL-21+ Th17 cells in PDAC: IL-21+ (red) & RORC+ (brown) cells. Black arrowhead: PDAC tumor cells, black arrow: IL-21+/RORC+ immune cells. (C) Examples of negative, weak & high expression of IL-21R (brown) & Blimp-1 (red) in human PDAC tissue. Black arrows: tumor cells. (D) Examples of IL-21R & Blimp-1 expression in healthy pancreas tissue. (E) IL-21R expression in human PDAC varied among the patients. (F) Association of IL-21R & Blimp-1 expression in human PDAC. * P < 0.05 & **** P < 0.0001. Unpaired t-test. (G) Co-expression of IL-21R (brown, membrane) & Blimp-1 (red, nucleus). Black arrowhead: IL-21R+/Blimp-1+, white arrow head: IL-21R+/Blimp-1−. Black bar: 20 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31540511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
IL-21R Antibody - BSA Free

Western Blot: IL-21R Antibody - BSA Free [NBP1-76739] -

Western Blot: IL-21R Antibody - BSA Free [NBP1-76739] - Western blot analysis of IL-21R in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 u/ml.
IL-21R Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] -

Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] - Immunofluorescence of IL21R in A431 cells with IL 21 Receptor antibody at 20 u/mL.

Green: IL-21 Receptor Antibody
Blue: DAPI staining
IL-21R Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] -

Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] - Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 u/mL.
IL-21R Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739] -

IL-21R expression in human PDAC cell lines. Untreated human PDAC cell lines AsPC-1, BxPC-3, and Panc-1 were tested for IL-21R expression in (A) immunofluorescence staining (left: IL-21R+ cells, right: Alexa488 goat anti-rabbit IgG only; nuclei: DAPI (blue), IL-21R: Alexa488 (green)) and (B) western blot. White bar: 20 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31540511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for IL-21R Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
An approximately 60 kDa band can be detected in Western Blot. Use in ICC/IF was reported in scientific literature (PMID: 31540511).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-21R

A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. Both IL-21R and the gammac are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

Long Name

Interleukin 21 Receptor

Alternate Names

CD360, IL-21 R, IL21R, NILR

Gene Symbol

IL21R

UniProt

Additional IL-21R Products

Product Documents for IL-21R Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IL-21R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-21R Antibody - BSA Free

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Protocols

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