IL-33 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76394

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IL-33 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide from near the center of human IL-33. The immunogen is located within amino acids 150 - 200 of IL-33. Amino Acid Squence: YESQHPSNESGDGVDGK

Specificity

Human IL-33 has 4 isoforms, including isoform 1 (270aa, 30.8kD), isoform 2 (228aa, 25.9kD), isoform 3 (228aa, 26.1kD) and isoform 4 (144aa, 16.3kD). This antibody detects only human isoform L. Mouse IL-33 has 1 isoform (266aa, 30kD). Rat IL-33 has only one isoform identified so far (264aa, 29.5kD).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-33 Antibody - BSA Free

Western Blot: IL-33 AntibodyBSA Free [NBP1-76394]

Western Blot: IL-33 AntibodyBSA Free [NBP1-76394]

Western Blot: IL-33 Antibody [NBP1-76394] - Loading: 15ug of human lung lysate. Antibodies: IL-33, 2 g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IL-33 Antibody - BSA Free

Western Blot: IL-33 Antibody - BSA Free [NBP1-76394] -

Western Blot: IL-33 Antibody - BSA Free [NBP1-76394] - Validation of IL-33 in Human Lymph Node.Loading: 15. ug of human lymph node lysateAntibodies: IL-33, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: IL-33 AntibodyBSA Free [NBP1-76394]

Western Blot: IL-33 AntibodyBSA Free [NBP1-76394]

Western Blot: IL-33 Antibody [NBP1-76394] - Analysis of IL-33 in A-20 cell lysate with IL-33 antibody at (A) 1 and (B) 2 ug/mL.
Western Blot: IL-33 AntibodyBSA Free [NBP1-76394]

Western Blot: IL-33 AntibodyBSA Free [NBP1-76394]

Western Blot: IL-33 Antibody [NBP1-76394] - Loading: 5ng (1) and 25ng (2) of IL-33 partial human recombinant protein.Antibodies: IL-33, NBP1-76394, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Il-33 partial human recombinant protein: 112-270aa, 17.9kD, 36kD band is the dimer.
Western Blot: IL-33 AntibodyBSA Free [NBP1-76394]

Western Blot: IL-33 AntibodyBSA Free [NBP1-76394]

Western Blot: IL-33 Antibody [NBP1-76394] - Regulated Expression Validation of IL-33 in Human Skin Fibroblastsexpression of IL-33 in human skin fibroblasts following alpha-irradiation were detected by anti-IL-33 antibodies. IL-33 levels were increased after Alpha-irradiation, which were inhibited by treatment of PPP.
Western Blot: IL-33 AntibodyBSA Free [NBP1-76394]

Western Blot: IL-33 AntibodyBSA Free [NBP1-76394]

Western Blot: IL-33 Antibody [NBP1-76394] - Human IL-33 has 4 isoforms, including isoform 1 (270aa, 30.8kD), isoform 2 (228aa, 25.9kD), isoform 3 (228aa, 26.1kD) and isoform 4 (144aa, 16.3kD). This antibody detects only human isoform L. Mouse IL-33 has 1 isoform (266aa, 30kD). Rat IL-33 has only one isoform identified so far (264aa, 29.5kD).
Immunohistochemistry-Paraffin: IL-33 Antibody - BSA Free [NBP1-76394]

Immunohistochemistry-Paraffin: IL-33 Antibody - BSA Free [NBP1-76394]

Immunohistochemistry-Paraffin: IL-33 Antibody [NBP1-76394] - Analysis of of IL-33 in human lymph node tissue with IL-33 antibody at 2.5 ug/ml.
IL-33 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IL-33 Antibody - BSA Free [NBP1-76394] -

Immunocytochemistry/ Immunofluorescence: IL-33 Antibody - BSA Free [NBP1-76394] - Validation of IL-33 in Human Lymph Node.Immunofluorescent analysis of 4% paraformaldehyde-fixed human lymph node tissue labeling IL-33 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for IL-33 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 29329586).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-33

Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1alpha/beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kappaB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Entrez Gene IDs

90865 (Human); 77125 (Mouse); 361749 (Rat)

Gene Symbol

IL33

UniProt

O95760

Additional IL-33 Products

Product Documents for IL-33 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IL-33 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-33 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-33 Antibody - BSA Free

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  • Q: Would you tell us the isotype of this antibody? Is it IgG?

    A: It is affinity chromatography purified via peptide column. The isotype is IgG.

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