Key Product Details

Species Reactivity

Human

Applications

ELISA, Enzyme-linked Immunosorbent Spot, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # B-E8

Format

Azide and BSA Free
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human IL-6

Specificity

recognizes both natural and recombinant human IL-6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Stable at 4C for 12 months.

Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells

Scientific Data Images for IL-6 Antibody (B-E8) - Azide and BSA Free

Flow Cytometry: IL-6 Antibody (B-E8) - Azide and BSA Free [NBP3-14588]

Flow Cytometry: IL-6 Antibody (B-E8) - Azide and BSA Free [NBP3-14588]

Flow Cytometry: IL-6 Antibody (B-E8) [NBP3-14588] - B-E8 with non-activated monocytes. In the two cases, the dotted line represents the IgG1 isotype control.
Flow Cytometry: IL-6 Antibody (B-E8) - Azide and BSA Free [NBP3-14588]

Flow Cytometry: IL-6 Antibody (B-E8) - Azide and BSA Free [NBP3-14588]

Flow Cytometry: IL-6 Antibody (B-E8) [NBP3-14588] - Activated monocytes marked with IL-6 antibody.

Applications for IL-6 Antibody (B-E8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Enzyme-linked Immunosorbent Spot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Biological Activity: Inhibits IL-6 induced proliferation on XG1 Cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS. Sterile-filtered through 0.22 um.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-6

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for IL-6 Antibody (B-E8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IL-6 Antibody (B-E8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 Antibody (B-E8) - Azide and BSA Free

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  • Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?

    A: We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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