Rabbit IL-6 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-62858

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rabbit

Specificity

This kit recognizes Rabbit IL-6 in samples. No significant cross-reactivity or interference between Rabbit IL-6 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.27%

Inter-Assay Precision (Precision between assays) CV% 5.45%

Recovery for Rabbit IL-6 ELISA Kit (Colorimetric)

Recovery

88-109%

Linearity

Rabbit IL-6 ELISA Kit (Colorimetric)

Scientific Data Images for Rabbit IL-6 ELISA Kit (Colorimetric)

Rabbit IL-6 ELISA Kit (Colorimetric)

ELISA: Rabbit IL-6 ELISA Kit (Colorimetric) [NBP2-62858] -

ELISA: Rabbit IL-6 ELISA Kit (Colorimetric) [NBP2-62858] - Standard Curve Reference

Kit Contents for Rabbit IL-6 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-6

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for Rabbit IL-6 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rabbit IL-6 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Rabbit IL-6 ELISA Kit (Colorimetric)

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  • Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?

    A: We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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