Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80818

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # P4C10

Format

Azide and BSA Free

View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

HT1080 cells human fibrosarcoma

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27550917).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free (NBP2-36561) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]

Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively. Image from the standard format of this antibody.

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals). Image from the standard format of this antibody.

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] Image from the standard format of this antibody.

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Applications for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1:10

Immunocytochemistry/ Immunofluorescence

1:10-1:100

Immunohistochemistry-Frozen

1:10-1:100

Western Blot

reported in scientific literature (PMID 27550917)

Application Notes

For ICC/IF,methanol fixation is recommended. The P4C10 antibody inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion.

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 1/CD29

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms complexes with at least 18 alpha and 8 beta subunits in mammals. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Integrins can be found on the cell membrane, melanosome, cleavage furrows, and lamellipodium on a subcellular level. Expression levels in tissues are dependent on the isoform.

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free

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Product Specific Notices for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules

Adhesion Molecules on Endothelial Cells

Adipose-derived Stem Cell Markers

Cancer Biomarkers

Cardiac Stem Cell Markers

Epithelial Cell Polarity

Integrins and Associated Molecules

Mesenchymal Stem Cell Markers

Skin Stem Cell Markers

Citations for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free

Showing 1 - 1 of 1 FAQ Showing All

Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.