Integrin beta 1/CD29 Antibody - BSA Free

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] -

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] -

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug whole cell lysate/extract of human Integrin beta 1 / CD29-transfected 293T cells
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (NBP2-16974) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (NBP2-16974) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (NBP2-16974) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody - BSA Free [NBP2-16974] -

Panels of confocal micrographs presenting the immunofluorescence staining of BME-UV1 cells cultured on Matrigel for 11 days in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Cells were stained with primary antibodies against beta 1-integrin and secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); Alexa Fluor 594 phalloidin, detecting F-actin (red fluorescence), and nuclei counterstained with Hoechst 33342 (blue fluorescence). Images were taken at 600× magnification and are representative for three independent experiments. Scale bar: 20 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/8/4147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.