JNK1 [p Thr183, p Tyr185] Antibody (HL1008) - Azide and BSA Free

Name: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-13678
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-13678

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1008 expressed in HEK293

Format

Azide and BSA Free

View all JNK1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr183/Tyr185 of human JNK1. The exact sequence is proprietary.

Modification

p Thr183, p Tyr185

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for JNK1 [p Thr183, p Tyr185] Antibody (HL1008) - Azide and BSA Free

Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - JNK1 (phospho Thr183/Tyr185) antibody [HL1008] detects JNK1 (phospho Thr183/Tyr185) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. JNK1 (phospho Thr183/Tyr185) stained by JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] -

Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] -

Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) Raw264.7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] -

Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) PC-12 whole cell extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] -

Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

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Applications for JNK1 [p Thr183, p Tyr185] Antibody (HL1008) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JNK1

JNK1, also known as MAPK1, is a JNK protein subgroup familymenber of the mitogen-activated protein kinase (MAPK) superfamily and is part of a signal transduction cascade that is activated by cytokines and by exposure to environmental stresses. Components of the JNK (c-Jun N-terminal kinase) signaling pathway have been shown to interact with the insulin signaling pathway and play a pivotal role in lifespan regulation and stress resistance in many diverse organisms. Therefore, JNK1 plays a role in regulating both life span and stress resistance.

Long Name

C-Jun N-terminal Kinase 1

Alternate Names

MAPK8, PRKM8, SAPK1

Gene Symbol

MAPK8

Additional JNK1 Products

Product Documents for JNK1 [p Thr183, p Tyr185] Antibody (HL1008) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for JNK1 [p Thr183, p Tyr185] Antibody (HL1008) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.