MEK1 Antibody (9B6) - BSA Free

Name: MEK1 Antibody (9B6) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-26153
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-26153

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9B6 expressed in HEK293

Format

BSA Free

View all MEK1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human MEK1 [UniProt Q02750]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEK1 Antibody (9B6) - BSA Free

Immunoprecipitation: MEK1 Antibody (9B6) [NBP3-26153] -

Immunoprecipitation: MEK1 Antibody (9B6) [NBP3-26153] - Immunoprecipitating MEK1 in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26153 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26153 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).

Immunohistochemistry: MEK1 Antibody (9B6) [NBP3-26153] -

Immunohistochemistry: MEK1 Antibody (9B6) [NBP3-26153] - Image of MEK1 Antibody (9B6) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: MEK1 Antibody (9B6) [NBP3-26153] -

Western Blot: MEK1 Antibody (9B6) [NBP3-26153] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, Rat brain tissue.
All lanes: MEK1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 44, 41 kDa
Observed band size: 44 kDa

Applications for MEK1 Antibody (9B6) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: MEK1

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

MAP2K1, MKK1

Gene Symbol

MAP2K1

Additional MEK1 Products

Product Documents for MEK1 Antibody (9B6) - BSA Free

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Product Specific Notices for MEK1 Antibody (9B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.