MEK1 Antibody - BSA Free

Name: MEK1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-87790
Price: 269 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-87790

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQ

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25408231).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEK1 Antibody - BSA Free

Western Blot: MEK1 Antibody [NBP1-87790]

Western Blot: MEK1 Antibody [NBP1-87790] - Analysis in human cell line U-87 MG.

Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP1-87790]

Immunocytochemistry/Immunofluorescence: MEK1 Antibody [NBP1-87790] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

Immunohistochemistry: MEK1 Antibody [NBP1-87790]

MEK1-Antibody-Immunohistochemistry-NBP1-87790-img0008.jpg

Immunohistochemistry-Paraffin: MEK1 Antibody [NBP1-87790]

Immunohistochemistry-Paraffin: MEK1 Antibody [NBP1-87790] - Staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.

Immunohistochemistry: MEK1 Antibody [NBP1-87790] -

Correlation between MEK1 expression and survival after platinum treatment in EOC patients. Expression measured by qRT-PCR: relapse-free survival of 34 patients with low and high MEK1 expressing tumors (A). Expression tested with IHC: overall survival of 59 independent patients with low and high MEK1 expression (B). Representative images of immunohistochemistry, low and high expression of MEK1 at low and high magnifications (C).

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Applications for MEK1 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml

Application Notes

IHC reported in scientific literature (PMID: 25408231). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEK1

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

MAP2K1, MKK1

Gene Symbol

MAP2K1

Additional MEK1 Products

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Product Specific Notices for MEK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.