MEK1 Antibody

Name: MEK1 Antibody
Brand: Novus Biologicals
SKU: NBP2-17265
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-17265

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Porcine

Predicted:

Bovine (100%), Canine (100%), Chicken (98%), Primate (98%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (96%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all MEK1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK1. The exact sequence is proprietary.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 31757483).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK1 Antibody

Western Blot: MEK1 Antibody [NBP2-17265]

Western Blot: MEK1 Antibody [NBP2-17265] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE gel, diluted at 1:2000.

Immunohistochemistry-Paraffin: MEK1 Antibody [NBP2-17265]

Immunohistochemistry-Paraffin: MEK1 Antibody [NBP2-17265] - Paraffin-embedded rat liver. MEK1+2 antibody diluted at 1:500.

Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP2-17265] -

Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP2-17265] - MEK1 antibody detects MEK1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEK1 stained by MEK1 antibody (NBP2-17265) diluted at 1:500.
Blue: Hoechst 33342 staining.

Western Blot: MEK1 Antibody [NBP2-17265] -

Western Blot: MEK1 Antibody [NBP2-17265] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (NBP2-17265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: MEK1 Antibody [NBP2-17265] -

Western Blot: MEK1 Antibody [NBP2-17265] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (NBP2-17265) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/ Immunofluorescence: MEK1 Antibody [NBP2-17265] -

MEK1+2 antibody detects MEK1+2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MEK1+2 protein stained by MEK1+2 antibody (NBP2-17265) diluted at 1:500.
Blue: Hoechst 33342 staining.

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Applications for MEK1 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MEK1

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq]

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

MAP2K1, MKK1

Gene Symbol

MAP2K1

UniProt

Q02750

Additional MEK1 Products

Product Documents for MEK1 Antibody

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Certificate of Analysis

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Product Specific Notices for MEK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.