MEK1 Antibody - Azide and BSA Free

Name: MEK1 Antibody - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-02951
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-02951

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEK1 (NP_002746.1). MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEK1 Antibody - Azide and BSA Free

Western Blot: MEK1 AntibodyAzide and BSA Free [NBP3-02951]

Western Blot: MEK1 Antibody [NBP3-02951] - Analysis of extracts from normal (control) and MAP2K1 knockout (KO) HeLa cells, using MEK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry

Immunohistochemistry-Paraffin: MEK1 Antibody - Azide and BSA Free [NBP3-02951]

Immunohistochemistry-Paraffin: MEK1 Antibody [NBP3-02951] - Paraffin-embedded mouse kidney using MEK1 antibody at dilution of 1:100 (40x lens).

Western Blot: MEK1 AntibodyAzide and BSA Free [NBP3-02951]

Western Blot: MEK1 Antibody [NBP3-02951] - Analysis of extracts of various cell lines, using MEK1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit

Immunohistochemistry: MEK1 Antibody - Azide and BSA Free [NBP3-02951]

Immunohistochemistry: MEK1 Antibody [NBP3-02951] - Paraffin-embedded Rat brain using MEK1 antibody at dilution of 1:100 (40x lens).

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Applications for MEK1 Antibody - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:1000-1:3000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK1

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

MAP2K1, MKK1

Gene Symbol

MAP2K1

Additional MEK1 Products

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Product Specific Notices for MEK1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov