MEK2 Antibody (OTI1A2)

Name: MEK2 Antibody (OTI1A2)
Brand: Novus Biologicals
SKU: NBP2-45802
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-45802

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # OTI1A2

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Product Specifications

Immunogen

Full length human recombinant protein of human MAP2K2(NP_109587) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Theoretical MW

44.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK2 Antibody (OTI1A2)

Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802]

Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802]

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802]

Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2.

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802]

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802]

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802]

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802]

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802]

Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunofluorescence: MEK2 Antibody (OTI1A2) [NBP2-45802]

Immunofluorescence: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MEK2.

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Applications for MEK2 Antibody (OTI1A2)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK2

MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Long Name

Mitogen-activated Protein Kinase Kinase 2

Alternate Names

MAP2K2, MKK2

Gene Symbol

MAP2K2

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Product Documents for MEK2 Antibody (OTI1A2)

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Product Specific Notices for MEK2 Antibody (OTI1A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.