MEK2 Antibody - Azide and BSA Free

Name: MEK2 Antibody - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-02952
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-02952

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

View all MEK2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEK2 (NP_109587.1). MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMAR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK2 Antibody - Azide and BSA Free

Western Blot: MEK2 AntibodyAzide and BSA Free [NBP3-02952]

Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of HeLa cells, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western Blot: MEK2 AntibodyAzide and BSA Free [NBP3-02952]

Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of various cell lines, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] -

Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] MEK2 Rabbit pAb (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] -

Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] - Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit pAb (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Applications for MEK2 Antibody - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:100-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK2

MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Long Name

Mitogen-activated Protein Kinase Kinase 2

Alternate Names

MAP2K2, MKK2

Gene Symbol

MAP2K2

Additional MEK2 Products

Product Documents for MEK2 Antibody - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for MEK2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov