Mouse alpha-Fetoprotein/AFP Antibody (870826)

R&D Systems | Catalog # MAB5369

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 870826
View all alpha-Fetoprotein/AFP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse alpha-Fetoprotein/AFP
Lys19-Val605
Accession # P02772

Specificity

Detects mouse alpha-Fetoprotein in direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse alpha-Fetoprotein/AFP Antibody (870826)

Mouse alpha ‑Fetoprotein/AFP ELISA Standard Curve.

Recombinant Mouse alpha ‑Fetoprotein/AFP protein was serially diluted 2-fold and captured by Rat Anti-Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Mouse alpha-Fetoprotein / AFP ELISA Standard Curve

Mouse alpha-Fetoprotein / AFP ELISA Standard Curve

Mouse alpha-Fetoprotein/AFP was serially diluted and captured by Rat Anti-Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse alpha-Fetoprotein/AFP Antibody (870826)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Mouse alpha ‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse alpha-Fetoprotein/AFP DuoSet ELISA Kit (Catalog # DY5369-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-Fetoprotein/AFP

AFP ( alpha -Fetoprotein) is a 69‑73 kDa member of the ALB/AFP/VDB family of proteins. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature mouse AFP is 587 amino acids (aa) in length. It contains three albumin domains (aa 20‑201, 208‑393 and 400‑591), plus 15 intrachain disulfide bonds. Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37 kDa form. Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform. Over aa 19‑605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human); 11576 (Mouse); 24177 (Rat)

Gene Symbol

AFP

UniProt

P02772

Additional alpha-Fetoprotein/AFP Products

Product Documents for Mouse alpha-Fetoprotein/AFP Antibody (870826)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse alpha-Fetoprotein/AFP Antibody (870826)

For research use only

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