Mouse alpha-Fetoprotein/AFP Antibody

R&D Systems | Catalog # AF5369

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all alpha-Fetoprotein/AFP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse alpha ‑Fetoprotein/AFP
Lys19-Val605
Accession # P02772

Specificity

Detects mouse alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with human alpha ‑Fetoprotein is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse alpha-Fetoprotein/AFP Antibody

Detection of Mouse a-Fetoprotein/AFP antibody by Western Blot.

Detection of Mouse alpha -Fetoprotein/AFP by Western Blot.

Western blot shows lysates of mouse plasma and rat placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Mouse alpha-Fetoprotein/AFP by Western Blot

Detection of Mouse alpha-Fetoprotein/AFP by Western Blot

Extensive hepatocyte damage by hepatocyte-specific activation of FOXO3 associated with upregulation of HCC-concomitant factors. a More inflammation can be observed in livers from 9-week-old FOXO3CAHep transgenic mice than in control animals. b Expression of Trp53 and Myc by qPCR in livers from 9-week-old control and FOXO3CAHep transgenic mice. c Western blot analysis in livers from 9-week-old control and FOXO3CAHep mice with p53 and c-Myc and GAPDH as loading control (left panel), as well as densitometric quantification (middle and right panels). d Expression of Afp by qPCR in livers from 9-week-old control and FOXO3CAHep transgenic mice (n = 4). e Representative IHC staining for AFP (bar = 100 μm).f Western blot analysis in livers from 9-week-old control and FOXO3CAHep mice with AFP and GAPDH as loading control (upper panel), as well as densitometric quantification (lower panel). g Serum AFP levels in 9-week-old control and FOXO3CAHep mice Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31488102), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse alpha-Fetoprotein/AFP by Western Blot

Detection of Mouse alpha-Fetoprotein/AFP by Western Blot

Enhanced proliferation and elevated expression of AFP and Vimentin in livers of DEN-injected 40-week-old FOXO3CAHep transgenic mice. a Representative IHC staining for Ki67, AFP, p53, E-Cadherin, N-Cadherin, and Vimentin in 40-week-old DEN-injected control and DEN-injected FOXO3CAHep transgenic mice (bar = 100 μm). b Scoring of Ki67-positivity (n = 4). c Western blot analysis in livers from 40-week-old DEN-injected control and DEN-injected FOXO3CAHep transgenic mice with AFP, c-Myc, p53, E-Cadherin, N-Cadherin, Vimentin and GAPDH as loading control (upper part), as well as densitometric quantification (lower part) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31488102), licensed under a CC-BY license. Not internally tested by R&D Systems.
Mouse alpha-Fetoprotein / AFP ELISA Standard Curve

Mouse alpha-Fetoprotein / AFP ELISA Standard Curve

Mouse/Rat IGF-I was serially diluted and captured by Rat Anti-Mouse ?‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ?‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Mouse alpha-Fetoprotein / AFP ELISA Standard Curve

Mouse alpha-Fetoprotein / AFP ELISA Standard Curve

Mouse/Rat IGF-I was serially diluted and captured by Rat Anti-Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse alpha-Fetoprotein/AFP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse plasma and rat placenta tissue

Reviewed Applications

Read 1 review rated 4 using AF5369 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-Fetoprotein/AFP

AFP ( alpha -Fetoprotein) is a 69‑73 kDa member of the ALB/AFP/VDB family of proteins. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature mouse AFP is 587 amino acids (aa) in length. It contains three albumin domains (aa 20‑201, 208‑393 and 400‑591), plus 15 intrachain disulfide bonds. Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37 kDa form. Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform. Over aa 19‑605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human); 11576 (Mouse); 24177 (Rat)

Gene Symbol

AFP

UniProt

P02772

Additional alpha-Fetoprotein/AFP Products

Product Documents for Mouse alpha-Fetoprotein/AFP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse alpha-Fetoprotein/AFP Antibody

For research use only

Citations for Mouse alpha-Fetoprotein/AFP Antibody

Customer Reviews for Mouse alpha-Fetoprotein/AFP Antibody (1)

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  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 24113455
    Species: Mouse
    Verified Customer | Posted 01/08/2015

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