Mouse ASGR1/ASGPR1 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse ASGPR1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse ASGPR1
    Ser60-Asn284
    Accession # Q91Y84
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 1.5-5 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated  beta ‑Galactosamine-N-Acetyl-Polyacrylamide to immobilized Recombinant Mouse ASGPR1 (Catalog # 2755-AS) coated at 2.5 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse ASGR1/ASGPR1 by Western Blot. Western blot shows lysate of mouse liver tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ASGR1/ASGPR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASGR1/ASGPR1 at approximately 40-46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ASGR1/ASGPR1

The mouse asialoglycoprotein receptor (ASGP-R) is an endocytic recycling receptor that belongs to the long-form subfamily of the C-type/Ca++-dependent lectin family (1-3). It is a complex of two noncovalently-linked subunits, a major 42 kDa glycoprotein (ASGPR1) and a minor 51 kDa glycoprotein (ASGR2). The major mouse ASGP-R subunit, ASGPR1, is synthesized as a 284 amino acid (aa) type II transmembrane (TM) protein that contains a 39 aa cytoplasmic region, a 21 aa TM segment, and a 224 aa extracellular domain (ECD) (4‑6). The ECD contains two important structural regions. The first is a stalk region of 56 aa (aa’s # 59-117) that contributes to noncovalent oligomerization. The second is a 118 aa, carbohydrate-binding, Ca++-dependent C-type lectin domain (aa’s 160-277) that is unusually stabilized by three Ca++ ions (3, 5). There are two potential alternate splice forms for ASGPR1. Both are TM and show a deletion of the C-type lectin domain. One is 113 aa in length and shows a deletion of aa’s # 114-284 (7). The second is 132 aa in length and shows a deletion of aa’s 118-146 and aa’s 162 - 284 (8). Mouse ASGPR1 ECD is 89% and 79% aa identical to the ASGPR1 ECD in rat and human, respectively. The minor mouse ASGP-R subunit, ASGR2, is also a C-type lectin that shares the same structural organization as ASGR-1. It is 301 aa in length and has two 45 kDa and 51 kDa differentially-glycosylated isoforms (4, 6, 9). The ECD of ASGR2 is 50% aa identical to the ECD of ASGPR1. Although ASGPR1 and 2 can be expressed individually, a fully functional and stable ASGP-R requires simultaneous expression of both subunits (10-12). The stoichiometry of a functional ASGP-R is suggested to be either a 2:2, 3:1 or 3:2 ratio of ASGPR1:ASGR2 (13, 14). ASGPR1 is reported to bind Gal (non-reducing), GalNAc, and sialic acid alpha 2,6GalNAc (3, 15, 16). This is generally in the context of triantennary or tetraantennary configurations (2).

  • References:
    1. Stockert, R. J. (1995) Physiol. Rev. 75:591.
    2. Weigel, P.H. and J.H.N. Yik (2002) Biochim. Biophys. Acta 1572:341.
    3. Meier, M. et al. (2000) J. Mol. Biol. 300:857.
    4. Takezawa, R. et al. (1993) Biochim. Biophys. Acta 1172 :220.
    5. Monroe, R.S. and B.E. Huber (1994) Gene 148:237.
    6. Sanford, J.P. et al. (1988) DNA 7:721.
    7. Heath, P. (2005) GenBank Accession # Q5NCV2.
    8. Heath, P. (2005) GenBank Accession # Q5NCV1.
    9. Sanford, J.P. and D. Doyle (1990) Biochim. Biophys. Acta 1087:259.
    10. Braun, J.R. et al. (1996) J. Biol. Chem. 271:21160.
    11. Ishibashi, S. et al. (1994) J. Biol. Chem. 269:27803.
    12. Tozawa, R. et al. (2001) J. Biol. Chem. 276:12624.
    13. Bider, M.D. et al. (1996) J. Biol. Chem. 271:31996.
    14. Lodish, H. (1991) Trends Biochem. Sci. 16:374.
    15. Westerlind, U. et al. (2004) Glyconj. J. 21:227.
    16. Park, E.I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17125.
  • Long Name:
    Asialoglycoprotein Receptor 1
  • Entrez Gene IDs:
    432 (Human); 11889 (Mouse); 24210 (Rat)
  • Alternate Names:
    ASGPR 1; ASGP-R 1; ASGPR; ASGPR1; ASGR1; asialoglycoprotein receptor 1; CLEC4H1; CLEC4H1ASGPR1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1; MHL1; RHL1
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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