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Mouse ASGR1/ASGPR1 Antibody

Catalog #: AF2755
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2755
AF2755-SP

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Detection of Mouse ASGR1/ASGPR1 by Western Blot.
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Mouse ASGR1/ASGPR1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ASGPR1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ASGPR1
Ser60-Asn284
Accession # Q91Y84
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1.5-5 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated  beta ‑Galactosamine-N-Acetyl-Polyacrylamide to immobilized Recombinant Mouse ASGPR1 (Catalog # 2755-AS) coated at 2.5 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse ASGR1/ASGPR1 antibody by Western Blot. View Larger

Detection of Mouse ASGR1/ASGPR1 by Western Blot. Western blot shows lysate of mouse liver tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ASGR1/ASGPR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASGR1/ASGPR1 at approximately 40-46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASGR1/ASGPR1

The mouse asialoglycoprotein receptor (ASGP-R) is an endocytic recycling receptor that belongs to the long-form subfamily of the C-type/Ca++-dependent lectin family (1-3). It is a complex of two noncovalently-linked subunits, a major 42 kDa glycoprotein (ASGPR1) and a minor 51 kDa glycoprotein (ASGR2). The major mouse ASGP-R subunit, ASGPR1, is synthesized as a 284 amino acid (aa) type II transmembrane (TM) protein that contains a 39 aa cytoplasmic region, a 21 aa TM segment, and a 224 aa extracellular domain (ECD) (4‑6). The ECD contains two important structural regions. The first is a stalk region of 56 aa (aa’s # 59-117) that contributes to noncovalent oligomerization. The second is a 118 aa, carbohydrate-binding, Ca++-dependent C-type lectin domain (aa’s 160-277) that is unusually stabilized by three Ca++ ions (3, 5). There are two potential alternate splice forms for ASGPR1. Both are TM and show a deletion of the C-type lectin domain. One is 113 aa in length and shows a deletion of aa’s # 114-284 (7). The second is 132 aa in length and shows a deletion of aa’s 118-146 and aa’s 162 - 284 (8). Mouse ASGPR1 ECD is 89% and 79% aa identical to the ASGPR1 ECD in rat and human, respectively. The minor mouse ASGP-R subunit, ASGR2, is also a C-type lectin that shares the same structural organization as ASGR-1. It is 301 aa in length and has two 45 kDa and 51 kDa differentially-glycosylated isoforms (4, 6, 9). The ECD of ASGR2 is 50% aa identical to the ECD of ASGPR1. Although ASGPR1 and 2 can be expressed individually, a fully functional and stable ASGP-R requires simultaneous expression of both subunits (10-12). The stoichiometry of a functional ASGP-R is suggested to be either a 2:2, 3:1 or 3:2 ratio of ASGPR1:ASGR2 (13, 14). ASGPR1 is reported to bind Gal (non-reducing), GalNAc, and sialic acid alpha 2,6GalNAc (3, 15, 16). This is generally in the context of triantennary or tetraantennary configurations (2).

References
  1. Stockert, R. J. (1995) Physiol. Rev. 75:591.
  2. Weigel, P.H. and J.H.N. Yik (2002) Biochim. Biophys. Acta 1572:341.
  3. Meier, M. et al. (2000) J. Mol. Biol. 300:857.
  4. Takezawa, R. et al. (1993) Biochim. Biophys. Acta 1172 :220.
  5. Monroe, R.S. and B.E. Huber (1994) Gene 148:237.
  6. Sanford, J.P. et al. (1988) DNA 7:721.
  7. Heath, P. (2005) GenBank Accession # Q5NCV2.
  8. Heath, P. (2005) GenBank Accession # Q5NCV1.
  9. Sanford, J.P. and D. Doyle (1990) Biochim. Biophys. Acta 1087:259.
  10. Braun, J.R. et al. (1996) J. Biol. Chem. 271:21160.
  11. Ishibashi, S. et al. (1994) J. Biol. Chem. 269:27803.
  12. Tozawa, R. et al. (2001) J. Biol. Chem. 276:12624.
  13. Bider, M.D. et al. (1996) J. Biol. Chem. 271:31996.
  14. Lodish, H. (1991) Trends Biochem. Sci. 16:374.
  15. Westerlind, U. et al. (2004) Glyconj. J. 21:227.
  16. Park, E.I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17125.
Long Name
Asialoglycoprotein Receptor 1
Entrez Gene IDs
432 (Human); 11889 (Mouse); 24210 (Rat)
Alternate Names
ASGPR 1; ASGP-R 1; ASGPR; ASGPR1; ASGR1; asialoglycoprotein receptor 1; CLEC4H1; CLEC4H1ASGPR1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1; MHL1; RHL1

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Citations for Mouse ASGR1/ASGPR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Hepatic PTP4A1 ameliorates high-fat diet-induced hepatosteatosis and hyperglycemia by the activation of the CREBH/FGF21 axis
    Authors: Byungtae Hwang, Min-Gi Kwon, Min Ji Cho, Nam-Kyung Lee, Jangwook Lee, Jeong Woong Lee et al.
    Theranostics
  2. Expression of Asialoglycoprotein Receptor 1 in Human Hepatocellular Carcinoma.
    Authors: Shi B, Abrams M, Sepp-Lorenzino L.
    J Histochem Cytochem

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