Mouse BMP-6 Antibody

Catalog # Availability Size / Price Qty
AF6325
AF6325-SP
Mouse BMP-6 Antibody in Western Blot
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Mouse BMP-6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects recombinant human and mouse BMP-6 in direct ELISAs and detects mouse BMP-6 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) BMP-5 and rmBMP-7 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse BMP-6
Ser372-His510
Accession # P20722
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse BMP‑6 by Western Blot. View Larger

Detection of Mouse BMP‑6 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with mouse BMP-6. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse BMP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6325) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Mouse BMP-6 (Catalog # 6325-BM) (10ng/lane) was included. A specific band was detected for BMP-6 at approximately 20kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry BMP‑6 in Mouse Embryo. View Larger

BMP‑6 in Mouse Embryo. BMP-6 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse BMP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6325) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to skeletal muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-6

Bone Morphogenetic Protein 6 (BMP‑6), also known as Vgr-1, is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor  beta (TGF-beta ) superfamily. Mouse BMP‑6 is synthesized as a 510 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C‑terminal mature protein. Biologically active BMP‑6 consists of a disulfide-linked homodimer of the mature proteins (1, 2). Mature mouse BMP‑6 shares 96% and 98% aa sequence identity with human and rat BMP‑6, respectively. Cellular responses to BMP‑6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3‑5). Glycosylation of BMP‑6 is required for its interaction with Activin RIA (6). BMP‑6 induces the expression of Noggin and is subsequently antagonized by Noggin (7, 8). BMP‑6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (5), chondrocyte maturation (9), Ang II-induced aldosterone production in the adrenal cortex (3), hormone production and responsiveness in ovarian granulosa cells (10), iNOS and TNF-alpha production in macrophages (4), the cell death of B cells (8), and neurite outgrowth (11). BMP‑6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). BMP‑6 is upregulated during prostate cancer progression and promotes tumor cell metastasis to bone (13). Through interactions with the BMP coreceptor RGM‑C/Hemojuvelin, BMP‑6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (14, 15).

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Lyons, K. et al. (1989) Proc. Natl. Acad. Sci. 86:4554.
  3. Inagaki, K. et al. (2006) Endocrinology 147:2681.
  4. Hong, J.H. et al. (2008) Immunology 128:e442.
  5. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  6. Saremba, S. et al. (2008) FEBS J. 275:172.
  7. Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
  8. Kersten, C. et al. (2005) BMC Immunol. 6:9.
  9. Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
  10. Shi, J. et al. (2009) Fertil. Steril. 92:1794.
  11. Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
  12. Zhang, Z. et al. (2006) Neuroscience 138:47.
  13. Dai, J. et al. (2005) Cancer Res. 65:8274.
  14. Meynard, D. et al. (2009) Nat. Genet. 41:478.
  15. Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.
Long Name
Bone Morphogenetic Protein 6
Entrez Gene IDs
654 (Human); 12161 (Mouse); 25644 (Rat)
Alternate Names
BMP6; BMP-6; bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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