Mouse c-Rel Antibody (290512)

Detection of Mouse c-Rel by Western Blot.

Western blot shows lysates of DA3 mouse myeloma cell line, A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse c-Rel Monoclonal Antibody (Catalog # MAB2699) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for c-Rel at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of c-Rel by Western Blot

HPV16 HPV E7 promotes nuclear factor kappa-light-chain-enhancer of activated B cell (NF-kappa B) activation in oral cells. (A) pHAGE/NF-kappa B reporter vector map. (B) Luciferase activity normalized with GFP was evaluated in SCC143/E7 and SCC143/V cells transfected with the reporter vector pHAGE/NF-kappa B. (C) Luciferase activity normalized with GFP in SCC143/E7 cells co-transfected with pHAGE/NF-kappa B and siRNA for PIR or siRNA E7 knockdown and siRNA (SCR) as a control. (D) Protein array of NF-kappa B signaling pathway comparing the SCC143/V and SCC143/E7 cell extracts. Data were plotted in reference to the change that occurred in the presence of E7 compared to the empty vector control. (E) Western blot of nuclear and cytoplasmic protein fractions was performed to analyze the levels of Pirin, p65S529, p65, and C-Rel. beta -actin or H3 were used as a load control in SCC143 cells transduced with empty (pLXSN) or E7 constructs. The graphs represent a densitometric analysis of three independent Western blots (WBs) for each protein normalized against beta -actin. Data are presented as the mean ± SEM; average of three independent experiments, conducted in triplicate. * p < 0.05 (Mann–Whitney test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32679705), licensed under a CC-BY license. Not internally tested by R&D Systems.