c-Rel is a member of the NF kappa B/Rel family of transcription factors. NF kappa B/Rel dimers containing c-Rel regulate expression of genes involved in lymphoid cell development, proliferation, and survival.
Mouse c‑Rel Biotinylated Antibody
R&D Systems | Catalog # BAM2699
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry
Cited:
Immunohistochemistry-Paraffin
Label
Biotin
Antibody Source
Monoclonal Rat IgG2A Clone # 290512
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Product Specifications
Immunogen
E. coli-derived recombinant mouse c-Rel
Met1-Ile588
Accession # P15307
Met1-Ile588
Accession # P15307
Specificity
Detects mouse c-Rel.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse c‑Rel Biotinylated Antibody
c‑Rel in Mouse Splenocytes.
c‑Rel was detected in immersion fixed mouse splenocytes using 10 µg/mL Mouse c‑Rel Biotinylated Monoclonal Antibody (Catalog # BAM2699) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse c‑Rel Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Reviewed Applications
Read 1 review rated 5 using BAM2699 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: c-Rel
Long Name
Cellular Repressor of E1A-stimulated Genes
Alternate Names
cRel, REL
Gene Symbol
REL
UniProt
Additional c-Rel Products
Product Documents for Mouse c‑Rel Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse c‑Rel Biotinylated Antibody
For research use only
Related Research Areas
Citations for Mouse c‑Rel Biotinylated Antibody
Customer Reviews for Mouse c‑Rel Biotinylated Antibody (1)
5 out of 5
1 Customer Rating
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Mouse splenocytesSpecies: MouseVerified Customer | Posted 02/25/2022
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars