Mouse CCL11/Eotaxin Biotinylated Antibody

(8 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL11/Eotaxin in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human CCL11/Eotaxin and recombinant mouse CCL24/Eotaxin-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CCL11/Eotaxin (R&D Systems, Catalog # 420-ME)
    His24-Pro97
    Accession # P48298
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse CCL11/Eotaxin Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse CCL11/Eotaxin Antibody (Catalog # AF-420-NA)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CCL11/Eotaxin Biotinylated Antibody (Catalog # BAF420)
  • ELISA Standard
     
    Recombinant Mouse CCL11/Eotaxin Protein (Catalog # 420-ME)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CCL11/Eotaxin in Mouse Colon. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF420) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.

  • References:
    1. Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
    2. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
    3. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
    4. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  • Entrez Gene IDs:
    6356 (Human); 20292 (Mouse)
  • Alternate Names:
    C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  2. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009;179(9):772-81.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  3. IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice.
    Authors: Wilson MS, Elnekave E, Mentink-Kane MM, Hodges MG, Pesce JT, Ramalingam TR, Thompson RW, Kamanaka M, Flavell RA, Keane-Myers A, Cheever AW, Wynn TA
    J. Clin. Invest., 2007;117(10):2941-51.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  4. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  5. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  6. A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
    Authors: Reading PC, Symons JA, Smith GL
    J. Immunol., 2003;170(3):1435-42.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  7. P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses.
    Authors: Larbi KY, Dangerfield JP, Culley FJ, Marshall D, Haskard DO, Jose PJ, Williams TJ, Nourshargh S
    J. Leukoc. Biol., 2003;73(1):65-73.
    Species: Mouse
    Sample Type: Peritoneal Fluid
    Application: ELISA Development
  8. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002;160(4):1353-60.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
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