CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.
Mouse CCL12/MCP-5 Antibody (63205)
R&D Systems | Catalog # MAB428
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 63205
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Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL12/MCP-5
Gly23-Gly104
Accession # Q62401.1
Gly23-Gly104
Accession # Q62401.1
Specificity
Detects mouse CCL12/MCP‑5 in ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL12/MCP-5 Antibody (63205)
Chemotaxis Induced by CCL12/MCP‑5 and Neutral-ization by Mouse CCL12/ MCP‑5 Antibody.
Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/ MCP-5 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP-5 Monoclonal Antibody (Catalog # MAB428). The ND50 is typically 0.5-2.5 µg/mL. Maximum neutralization observed was around 80%.
Mouse CCL12 / MCP-5 ELISA Standard Curve
Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5) was serially diluted and captured by Rat Anti-Mouse CCL12/MCP‑5 Monoclonal Antibody (Catalog # MAB428) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL12/MCP‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF428) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse CCL12/MCP-5 Antibody (63205)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5)
Sample: Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5)
Neutralization
Measured by its ability to neutralize CCL12/MCP‑5-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse CCL12/MCP‑5.
Mouse CCL12/MCP-5 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CCL12/MCP-5
References
- Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
- Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.
Alternate Names
MCP-5, MCP5
Entrez Gene IDs
20293 (Mouse)
Gene Symbol
CCL12
UniProt
Additional CCL12/MCP-5 Products
Product Documents for Mouse CCL12/MCP-5 Antibody (63205)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CCL12/MCP-5 Antibody (63205)
For research use only
Related Research Areas
Citations for Mouse CCL12/MCP-5 Antibody (63205)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
