Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 63205
View all CCL12/MCP-5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL12/MCP-5
Gly23-Gly104
Accession # Q62401.1

Specificity

Detects mouse CCL12/MCP‑5 in ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL12/MCP‑5 Antibody

Chemotaxis Induced by CCL12/MCP‑5 and Neutral-ization by Mouse CCL12/ MCP‑5 Antibody.

Chemotaxis Induced by CCL12/MCP‑5 and Neutral-ization by Mouse CCL12/ MCP‑5 Antibody.

Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/ MCP-5 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP-5 Monoclonal Antibody (Catalog # MAB428). The ND50 is typically 0.5-2.5 µg/mL. Maximum neutralization observed was around 80%.

Applications for Mouse CCL12/MCP‑5 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5)

Neutralization

Measured by its ability to neutralize CCL12/MCP‑5-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse CCL12/MCP‑5.

Mouse CCL12/MCP-5 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CCL12/MCP‑5 Biotinylated Antibody (Catalog # BAF428)
  • Standard: Recombinant Mouse CCL12/MCP-5 Protein (Catalog # 428-P5)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL12/MCP-5

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

References

  1. Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
  2. Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.

Alternate Names

MCP-5, MCP5

Entrez Gene IDs

20293 (Mouse)

Gene Symbol

CCL12

UniProt

Q62401.1

Additional CCL12/MCP-5 Products

Product Documents for Mouse CCL12/MCP‑5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL12/MCP‑5 Antibody

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Mouse CCL12/MCP‑5 Antibody

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