Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Neutralization, Flow Cytometry, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCL12/MCP-5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL12/MCP-5 (R&D Systems, Catalog # 428-P5)
Gly23-Gly104
Accession # Q62401

Specificity

Detects mouse CCL12/MCP-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) MCP‑3 is observed and 5% cross-reactivity with recombinant mouse (rm) JE, rmMARC, rhMCP‑1, and rhMCP‑2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL12/MCP‑5 Antibody

Chemotaxis Induced by CCL12/MCP‑5 and Neutralization by Mouse CCL12/MCP‑5 Antibody.

Chemotaxis Induced by CCL12/MCP‑5 and Neutralization by Mouse CCL12/MCP‑5 Antibody.

Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP‑5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL12/MCP‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF428). The ND50 is typically 0.8-4.0 µg/mL.

Applications for Mouse CCL12/MCP‑5 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5)

Neutralization

Measured by its ability to neutralize CCL12/MCP‑5-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.8-4.0 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse CCL12/MCP‑5.

Reviewed Applications

Read 1 review rated 4 using AF428 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL12/MCP-5

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

References

  1. Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
  2. Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.

Alternate Names

MCP-5, MCP5

Entrez Gene IDs

20293 (Mouse)

Gene Symbol

CCL12

UniProt

Q62401

Additional CCL12/MCP-5 Products

Product Documents for Mouse CCL12/MCP‑5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL12/MCP‑5 Antibody

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Mouse CCL12/MCP‑5 Antibody

Customer Reviews for Mouse CCL12/MCP‑5 Antibody (1)

4 out of 5
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  • Mouse CCL12/MCP-5 Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: fibroblasts
    Species: Mouse
    Verified Customer | Posted 11/29/2016
    Naïve cardiac fibroblasts were stimulated with conditioned media with or without Ccl12 blocking antibody at 10 μg/mL for 24 h

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