Mouse CCL19/MIP-3 beta Antibody Summary
Accession # Q548P0
under non-reducing conditions only
Mouse CCL19/MIP-3 beta Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL19/MIP‑3 beta and Neutral-ization by Mouse CCL19/MIP‑3 beta Antibody. Recombinant Mouse CCL19/ MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL19/ MIP-3 beta Monoclonal Antibody (Catalog # MAB880). The ND50 is typically 3-15 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL19/MIP-3 beta
CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a beta chemokine that binds specifically to the chemokine receptor CCR7/EBI-1/BLR-2. Mouse (human) CCL19 cDNA encodes a 108 (98) amino acid precursor protein with a predicted 25 (21) aa signal peptide that is cleaved to form the 83 (77) aa mature secreted protein. CCL19 is distantly related to other beta chemokines (20 - 30% aa sequence identity). Mouse CCL19 shares 83% aa sequence homology with human CCL19. CCL19 has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of CCL19 is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant CCL19 has been shown to be chemotactic for T-cells and B-cells. The CCL19 receptor (CCR-7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up‑regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
- Kim, C.H. et al. (1998) J. Immunol. 160:2418.
- Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
- Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
- Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Citations for Mouse CCL19/MIP-3 beta Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells
Authors: A Venkatesh, H Nandigam, M Muccioli, M Singh, T Loftus, D Lewis, M Pate, F Benencia
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
Thymic T cell development and progenitor localization depend on CCR7.
Authors: Misslitz A, Pabst O, Hintzen G, Ohl L, Kremmer E, Petrie HT, Forster R
J. Exp. Med., 2004;200(4):481-91.
Sample Types: Whole Tissue
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