Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Bioassay, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 87102
View all CCL19/MIP-3 beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL19/MIP-3 beta
Gly26-Val107-Leu-Glu (Ser108LeuGlu)
Accession # Q548P0

Specificity

Detects mouse CCL19/MIP‑3 beta  in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10, 11, 12, 17, 20, 21, 22, 24, 25, 27 or recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL19/MIP‑3 beta Antibody

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutral-ization by Mouse CCL19/MIP‑3 beta Antibody.

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutral-ization by Mouse CCL19/MIP‑3 beta Antibody.

Recombinant Mouse CCL19/ MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL19/ MIP-3 beta Monoclonal Antibody (Catalog # MAB880). The ND50 is typically 3-15 µg/mL.

Applications for Mouse CCL19/MIP‑3 beta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 50 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.

Mouse CCL19/MIP-3 beta Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CCL19/MIP‑3 beta Biotinylated Antibody (Catalog # BAF880)
  • Standard: Recombinant Mouse CCL19/MIP-3 beta Protein (Catalog # 440-M3)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL19/MIP-3 beta

CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a beta  chemokine that binds specifically to the chemokine receptor CCR7/EBI-1/BLR-2. Mouse (human) CCL19 cDNA encodes a 108 (98) amino acid precursor protein with a predicted 25 (21) aa signal peptide that is cleaved to form the 83 (77) aa mature secreted protein. CCL19 is distantly related to other beta chemokines (20 - 30% aa sequence identity). Mouse CCL19 shares 83% aa sequence homology with human CCL19. CCL19 has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of CCL19 is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant CCL19 has been shown to be chemotactic for T-cells and B-cells. The CCL19 receptor (CCR-7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up‑regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

References

  1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
  2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

Alternate Names

ELC, MIP-3 beta, MIP3 beta

Entrez Gene IDs

6363 (Human); 24047 (Mouse); 362506 (Rat)

Gene Symbol

CCL19

UniProt

Q548P0

Additional CCL19/MIP-3 beta Products

Product Documents for Mouse CCL19/MIP‑3 beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL19/MIP‑3 beta Antibody

For research use only

Citations for Mouse CCL19/MIP‑3 beta Antibody

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