Mouse CCL19/MIP-3 beta Antibody

Catalog # Availability Size / Price Qty
AF880
AF880-SP

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Chemotaxis Induced by CCL19/MIP‑3 beta  and Neutralization by Mouse CCL19/MIP‑3 beta  Antibody.
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Product Details
Citations (11)
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Mouse CCL19/MIP-3 beta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL19/MIP-3 beta in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL19/MIP-3 beta
Gly26-Val107 (Ser108LeuGlu)
Accession # Q548P0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis inthe BaF3 mouse pro-B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 0.8-4 μg/mL in the presence of 50 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Chemotaxis Induced by CCL19/MIP‑3 beta  and Neutralization by Mouse CCL19/MIP‑3 beta  Antibody. View Larger

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCL19/MIP‑3 beta Antibody. Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880). The ND50 is typically 0.8-4 µg/mL.

Immunocytochemistry CCL19/MIP-3 beta  antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CCL19/MIP‑3 beta in Mouse Splenocytes. CCL19/MIP-3 beta was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry CCL19/MIP‑3 beta  antibody in Mouse Dendritic Cells by Immunocytochemistry (ICC). View Larger

CCL19/MIP‑3 beta in Mouse Dendritic Cells. CCL19/MIP-3 beta was detected in immersion fixed mouse dendritic cells using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL19/MIP-3 beta

CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta  chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta  has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.

References
  1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
  2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Entrez Gene IDs
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

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Citations for Mouse CCL19/MIP-3 beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses
    Authors: V Saxena, W Piao, L Li, C Paluskievi, Y Xiong, T Simon, R Lakhan, CC Brinkman, S Walden, KL Hippen, M WillsonShi, YS Lee, C Wagner, BR Blazar, JS Bromberg
    Cell Reports, 2022;39(3):110727.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Type I interferon antagonism of the JMJD3-IRF4 pathway modulates macrophage activation and polarization
    Authors: K Ming-Chin, AA Achuthan, DP De Souza, TJ Lupancu, KJ Binger, MKS Lee, Y Xu, MJ McConville, NA de Weerd, D Dragoljevi, PJ Hertzog, AJ Murphy, JA Hamilton, AJ Fleetwood
    Cell Reports, 2022;39(3):110719.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense
    Authors: M Chen, RR Reed, AP Lane
    Cell Stem Cell, 2019;25(4):501-513.e5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. CCR7-CCL19/CCL21 Axis is Essential for Effective Arteriogenesis in a Murine Model of Hindlimb Ischemia
    Authors: AY Nossent, AJ Bastiaanse, EA Peters, MR de Vries, Z Aref, SM Welten, SC de Jager, TC van der Po, PH Quax
    J Am Heart Assoc, 2017;6(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
    Authors: Ibrahim M, Barnes J, Osorio E, Anstead G, Jimenez F, Osterholzer J, Travi B, Ahuja S, White A, Melby P
    Infect Immun, 2014;82(8):3098-112.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B.
    Authors: Jimenez F, Quinones MP, Martinez HG, Estrada CA, Clark K, Garavito E, Ibarra J, Melby PC, Ahuja SS
    J. Immunol., 2010;184(10):5571-81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. CCR7 modulates pulmonary and lymph node inflammatory responses in cigarette smoke-exposed mice.
    Authors: Demoor T, Bracke KR, Vermaelen KY, Dupont L, Joos GF, Brusselle GG
    J. Immunol., 2009;183(12):8186-94.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
    Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
    J. Immunol., 2009;182(11):6851-60.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo.
    Authors: Worbs T, Mempel TR, Bolter J, von Andrian UH, Forster R
    J. Exp. Med., 2007;204(3):489-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis.
    Authors: Ato M, Stager S, Engwerda CR, Kaye PM
    Nat. Immunol., 2002;3(12):1185-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Application: Func Sample Tested: Paraffined tumor tissue from HCC xenograft Species: Mouse