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Mouse CCL19/MIP-3 beta Antibody

Catalog #: AF880
16 Citations 1 Review Datasheet / COA / SDS
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AF880
AF880-SP

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Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCL19/MIP‑3 beta Antibody.
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Citations (16)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL19/MIP-3 beta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL19/MIP-3 beta in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL19/MIP-3 beta
Gly26-Val107 (Ser108LeuGlu)
Accession # Q548P0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis inthe BaF3 mouse pro-B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 0.8-4 μg/mL in the presence of 50 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCL19/MIP‑3 beta Antibody. View Larger

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCL19/MIP‑3 beta Antibody. Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880). The ND50 is typically 0.8-4 µg/mL.

Immunocytochemistry CCL19/MIP-3 beta antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CCL19/MIP‑3 beta in Mouse Splenocytes. CCL19/MIP-3 beta was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry CCL19/MIP‑3 beta antibody in Mouse Dendritic Cells by Immunocytochemistry (ICC). View Larger

CCL19/MIP‑3 beta in Mouse Dendritic Cells. CCL19/MIP-3 beta was detected in immersion fixed mouse dendritic cells using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL19/MIP-3 beta

CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta  chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta  has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.

References
  1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
  2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Entrez Gene IDs
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

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Citations for Mouse CCL19/MIP-3 beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Aligning cellular and molecular components in age-dependent tertiary lymphoid tissues of kidney and liver
    Authors: Toriu, N;Sato, Y;Kamimura, H;Yoshikawa, T;Tanaka, M;Yamamoto, S;Fukuma, S;Hattori, M;Terai, S;Yanagita, M;
    PloS one
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity
    Authors: J Liu, Y Cheng, X Zhang, Y Chen, H Zhu, K Chen, S Liu, Z Li, X Cao
    Cell Reports, 2023-01-18;42(1):111991.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses
    Authors: V Saxena, W Piao, L Li, C Paluskievi, Y Xiong, T Simon, R Lakhan, CC Brinkman, S Walden, KL Hippen, M WillsonShi, YS Lee, C Wagner, BR Blazar, JS Bromberg
    Cell Reports, 2022-04-19;39(3):110727.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Inflammation rapidly recruits mammalian GMP and MDP from bone marrow into regional lymphatics
    Authors: Juana Serrano-Lopez, Shailaja Hegde, Sachin Kumar, Josefina Serrano, Jing Fang, Ashley M Wellendorf et al.
    eLife
  5. Embryonic FAP+ lymphoid tissue organizer cells generate the reticular network of adult lymph nodes
    Authors: Alice E. Denton, Edward J. Carr, Lukasz P. Magiera, Andrew J.B. Watts, Douglas T. Fearon
    Journal of Experimental Medicine
  6. Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense
    Authors: M Chen, RR Reed, AP Lane
    Cell Stem Cell, 2019-09-12;25(4):501-513.e5.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC-Fr, Neutralization
  7. CCR7-CCL19/CCL21 Axis is Essential for Effective Arteriogenesis in a Murine Model of Hindlimb Ischemia
    Authors: AY Nossent, AJ Bastiaanse, EA Peters, MR de Vries, Z Aref, SM Welten, SC de Jager, TC van der Po, PH Quax
    J Am Heart Assoc, 2017-03-08;6(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney
    Authors: Yuki Sato, Akiko Mii, Yoko Hamazaki, Harumi Fujita, Hirosuke Nakata, Kyoko Masuda et al.
    JCI Insight
  9. Graft Site Microenvironment Determines Dendritic Cell Trafficking Through the CCR7-CCL19/21 Axis
    Authors: Jing Hua, William Stevenson, Thomas H. Dohlman, Takenori Inomata, Maryam Tahvildari, Narghes Calcagno et al.
    Investigative Opthalmology & Visual Science
  10. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
    Authors: Ibrahim M, Barnes J, Osorio E, Anstead G, Jimenez F, Osterholzer J, Travi B, Ahuja S, White A, Melby P
    Infect Immun, 2014-05-12;82(8):3098-112.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B.
    Authors: Jimenez F, Quinones MP, Martinez HG, Estrada CA, Clark K, Garavito E, Ibarra J, Melby PC, Ahuja SS
    J. Immunol., 2010-04-19;184(10):5571-81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. CCR7 modulates pulmonary and lymph node inflammatory responses in cigarette smoke-exposed mice.
    Authors: Demoor T, Bracke KR, Vermaelen KY, Dupont L, Joos GF, Brusselle GG
    J. Immunol., 2009-12-15;183(12):8186-94.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
    Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
    J. Immunol., 2009-06-01;182(11):6851-60.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo.
    Authors: Worbs T, Mempel TR, Bolter J, von Andrian UH, Forster R
    J. Exp. Med., 2007-02-26;204(3):489-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis.
    Authors: Ato M, Stager S, Engwerda CR, Kaye PM
    Nat. Immunol., 2002-11-18;3(12):1185-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Mouse CCL19/MIP‑3 beta Antibody
By Anonymous on 03/28/2018
Application: Func Sample Tested: Paraffined tumor tissue from HCC xenograft Species: Mouse