Mouse CCL19/MIP-3 beta Antibody

(8 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL19/MIP-3 beta in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant rat CCL19/MIP-3 beta is observed and less than 10% cross-reactivity with recombinant human CCL19/MIP‑3 beta is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CCL19/MIP-3 beta
    Gly26-Val107 (Ser108LeuGlu)
    Accession # Q548P0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.01 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3)
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis inthe BaF3 mouse pro-B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 0.8-4 μg/mL in the presence of 50 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCL19/MIP‑3 beta Antibody. Recombinant Mouse CCL19/MIP‑3 beta (Catalog #
440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line).The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP‑3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880). The ND50 is typically 0.8-4 μg/mL.
Immunocytochemistry
CCL19/MIP‑3 beta in Mouse Splenocytes. CCL19/MIP‑3 beta was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
CCL19/MIP‑3 beta in Mouse Dendritic Cells. CCL19/MIP‑3 beta was detected in immersion fixed mouse dendritic cells using Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL19/MIP-3 beta

CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta  chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta  has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.

  • References:
    1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
    2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
    3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
    4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
  • Entrez Gene IDs:
    6363 (Human); 24047 (Mouse); 362506 (Rat)
  • Alternate Names:
    beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. CCR7-CCL19/CCL21 Axis is Essential for Effective Arteriogenesis in a Murine Model of Hindlimb Ischemia
    Authors: AY Nossent, AJ Bastiaanse, EA Peters, MR de Vries, Z Aref, SM Welten, SC de Jager, TC van der Po, PH Quax
    J Am Heart Assoc, 2017;6(3):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  2. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
    Authors: Ibrahim M, Barnes J, Osorio E, Anstead G, Jimenez F, Osterholzer J, Travi B, Ahuja S, White A, Melby P
    Infect Immun, 2014;82(8):3098-112.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B.
    Authors: Jimenez F, Quinones MP, Martinez HG, Estrada CA, Clark K, Garavito E, Ibarra J, Melby PC, Ahuja SS
    J. Immunol., 2010;184(10):5571-81.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
    Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
    J. Immunol., 2009;182(11):6851-60.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. CCR7 modulates pulmonary and lymph node inflammatory responses in cigarette smoke-exposed mice.
    Authors: Demoor T, Bracke KR, Vermaelen KY, Dupont L, Joos GF, Brusselle GG
    J. Immunol., 2009;183(12):8186-94.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo.
    Authors: Worbs T, Mempel TR, Bolter J, von Andrian UH, Forster R
    J. Exp. Med., 2007;204(3):489-95.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  8. Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis.
    Authors: Ato M, Stager S, Engwerda CR, Kaye PM
    Nat. Immunol., 2002;3(12):1185-91.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh/Frozen
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