Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Neutralization, ELISA Development, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 158132
View all CCL22/MDC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # O88430

Specificity

Detects mouse CCL22/MDC in ELISAs and Western blots. In Western blots, this antibody shows 25% cross-reactivity with recombinant viral MIP‑II and no cross-reactivity with rmCCL1, 2, 3, 4, 6, 7, CCL9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 24, 25, 27, rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, or rrCCL20.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL22/MDC Antibody

Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody.

Mouse CCL22/MDC Antibody (Catalog # MAB4391) neutralizes CCL22/MDC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 1.00 - 12.0 μg/mL in the presence of 10.0 ng/mL Recombinant Mouse CCL22/MDC.

Applications for Mouse CCL22/MDC Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 1.00 - 12.0 μg/mL in the presence of 10.0 ng/mL Recombinant Mouse CCL22/MDC.

Mouse CCL22/MDC Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CCL22/MDC Biotinylated Antibody (Catalog # BAF439)
  • Standard: Recombinant Mouse CCL22/MDC Protein (Catalog # 439-MD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB4391 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL22/MDC

CCL22, also known as ABCD-1 and MDC (macrophage-derived chemokine), is a CC chemokine cloned from activated mouse B cells. Mouse CCL22 cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human CCL22 share 64% identity and 83% similarity. The genomic organization of the mouse and human CCL22 genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse CCL22 is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse CCL22 mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. CCL22 is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

References

  1. Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
  2. Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
  3. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
  4. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Alternate Names

ABCD-1, MDC, STCP-1

Entrez Gene IDs

6367 (Human); 20299 (Mouse)

Gene Symbol

CCL22

UniProt

O88430

Additional CCL22/MDC Products

Product Documents for Mouse CCL22/MDC Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CCL22/MDC Antibody

For research use only

Citations for Mouse CCL22/MDC Antibody

Customer Reviews for Mouse CCL22/MDC Antibody (1)

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  • Mouse CCL22/MDC Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 05/13/2021
    This antibody was used to coat the wells of a 96-well plate in conjunctions with BAF439 in an immunoassay for MDC.
    Mouse CCL22/MDC Antibody MAB4391

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