Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL24/Eotaxin‑2/MPIF‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 25, 28, or recombinant rat CCL20 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 106521
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
    Ile24-Val119
    Accession # Q9JKC0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 528-MP)
    • Mouse CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CCL24/Eotaxin‑2/MPIF‑2 Antibody (Catalog # MAB528)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody (Catalog # BAF528)
  • ELISA Standard
     
    Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119) Protein (Catalog # 528-MP)
  • Neutralization
    Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.06-0.3 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutral­ization by Mouse CCL24/
Eotaxin‑2 Antibody.
Recombinant Mouse CCL24/
Eotaxin‑2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin‑2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL24/Eotaxin‑2 Monoclonal Antibody (Catalog # MAB528). The ND50 is typically 0.06‑0.3 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to CCL11/Eotaxin and CCL26/Eotaxin-3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin.

  • Entrez Gene IDs:
    6369 (Human); 56221 (Mouse)
  • Alternate Names:
    CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
    Authors: Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson
    PLoS Pathog., 2017;13(7):e1006536.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. A critical role for IRF5 in regulating allergic airway inflammation
    Authors: AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova
    Mucosal Immunol, 2017;10(3):716-726.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
  3. IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice.
    Authors: Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N, Saito Y, Hatano M, Tokuhisa T, Iwakura Y, Puccetti P, Iwamoto I, Nakajima H
    Am. J. Respir. Crit. Care Med., 2008;178(10):1023-32.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
    Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
    Life Sci., 2005;78(9):987-94.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  5. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 0;43(5):945-58.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
Isotype Controls
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Rat IgG2A Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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