Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Neutralization, ELISA Development, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 106521
View all CCL24/Eotaxin-2/MPIF-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
Ile24-Val119
Accession # Q9JKC0

Specificity

Detects mouse CCL24/Eotaxin‑2/MPIF‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 25, 28, or recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL24/Eotaxin‑2/MPIF‑2 Antibody

Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutral-ization by Mouse CCL24/ Eotaxin‑2 Antibody.

Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutral-ization by Mouse CCL24/ Eotaxin‑2 Antibody.

Recombinant Mouse CCL24/ Eotaxin-2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin-2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL24/Eotaxin-2 Monoclonal Antibody (Catalog # MAB528). The ND50 is typically 0.06-0.3 µg/mL.

Applications for Mouse CCL24/Eotaxin‑2/MPIF‑2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 528-MP)

Neutralization

Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.06-0.3 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2.

Mouse CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody (Catalog # BAF528)
  • Standard: Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119) Protein (Catalog # 528-MP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to CCL11/Eotaxin and CCL26/Eotaxin-3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin.

Alternate Names

Eotaxin-2, MPIF-2, MPIF2

Entrez Gene IDs

6369 (Human); 56221 (Mouse)

Gene Symbol

CCL24

UniProt

Q9JKC0

Additional CCL24/Eotaxin-2/MPIF-2 Products

Product Documents for Mouse CCL24/Eotaxin‑2/MPIF‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL24/Eotaxin‑2/MPIF‑2 Antibody

For research use only

Citations for Mouse CCL24/Eotaxin‑2/MPIF‑2 Antibody

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