Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

Catalog #: MAB528
5 Citations Datasheet / COA / SDS

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MAB528-SP
MAB528-100
MAB528-500

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Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutral-ization by Mouse CCL24/ Eotaxin‑2 Antibody.

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Citations (5)

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Mouse CCL24/Eotaxin-2/MPIF-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL24/Eotaxin‑2/MPIF‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 25, 28, or recombinant rat CCL20 is observed.

Source

Monoclonal Rat IgG_2A Clone # 106521

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
Ile24-Val119
Accession # Q9JKC0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 528-MP)

Mouse CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody (Catalog # BAF528)

Standard: Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119) Protein (Catalog # 528-MP)

Neutralization

Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND₅₀) is typically 0.06-0.3 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutral-ization by Mouse CCL24/ Eotaxin‑2 Antibody. Recombinant Mouse CCL24/ Eotaxin-2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin-2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL24/Eotaxin-2 Monoclonal Antibody (Catalog # MAB528). The ND₅₀ is typically 0.06-0.3 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to CCL11/Eotaxin and CCL26/Eotaxin-3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin.

Entrez Gene IDs

6369 (Human); 56221 (Mouse)

Alternate Names

CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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A critical role for IRF5 in regulating allergic airway inflammation
Authors: AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova
Mucosal Immunol, 2017;10(3):716-726.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture)
TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
Authors: Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson
PLoS Pathog., 2017;13(7):e1006536.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice.
Authors: Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N, Saito Y, Hatano M, Tokuhisa T, Iwakura Y, Puccetti P, Iwamoto I, Nakajima H
Am. J. Respir. Crit. Care Med., 2008;178(10):1023-32.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
Life Sci., 2005;78(9):987-94.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development
Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
Immunity, 0;43(5):945-58.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture)