Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

Catalog # Availability Size / Price Qty
MAB528-SP
MAB528-100
MAB528-500
Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutral-ization by Mouse CCL24/ Eotaxin‑2 Antibody.
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Citations (8)
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Mouse CCL24/Eotaxin-2/MPIF-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL24/Eotaxin‑2/MPIF‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 25, 28, or recombinant rat CCL20 is observed.
Source
Monoclonal Rat IgG2A Clone # 106521
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
Ile24-Val119
Accession # Q9JKC0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 528-MP)

Mouse CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody (Catalog # BAF528)

Standard: Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119) Protein (Catalog # 528-MP)

Neutralization
Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.06-0.3 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutral-ization by Mouse CCL24/ Eotaxin‑2 Antibody. View Larger

Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutral-ization by Mouse CCL24/ Eotaxin‑2 Antibody. Recombinant Mouse CCL24/ Eotaxin-2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin-2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL24/Eotaxin-2 Monoclonal Antibody (Catalog # MAB528). The ND50 is typically 0.06-0.3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to CCL11/Eotaxin and CCL26/Eotaxin-3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin.

Entrez Gene IDs
6369 (Human); 56221 (Mouse)
Alternate Names
CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6

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Citations for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. ICOS signaling limits regulatory T cell accumulation and function in visceral adipose tissue
    Authors: Kristen L. Mittelsteadt, Erika T. Hayes, Daniel J. Campbell
    Journal of Experimental Medicine
  2. A CCL24-dependent pathway augments eosinophilic airway inflammation in house dust mite-challenged Cd163(-/-) mice
    Authors: Cuilian Dai, Xianglan Yao, Elizabeth M. Gordon, Amisha Barochia, Rosemarie A. Cuento, Maryann Kaler et al.
    Mucosal Immunology
  3. TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
    Authors: Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson
    PLoS Pathog., 2017-07-31;13(7):e1006536.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. A critical role for IRF5 in regulating allergic airway inflammation
    Authors: AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova
    Mucosal Immunol, 2016-10-19;10(3):716-726.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  5. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 2015-11-17;43(5):945-58.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  6. IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice.
    Authors: Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N, Saito Y, Hatano M, Tokuhisa T, Iwakura Y, Puccetti P, Iwamoto I, Nakajima H
    Am. J. Respir. Crit. Care Med., 2008-09-11;178(10):1023-32.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
    Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
    Life Sci., 2005-10-17;78(9):987-94.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  8. A20-binding inhibitor of NF-kappa B (ABIN) 2 negatively regulates allergic airway inflammation
    Authors: Sonia Ventura, Florencia Cano, Yashaswini Kannan, Felix Breyer, Michael J. Pattison, Mark S. Wilson et al.
    Journal of Experimental Medicine

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