Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

R&D Systems | Catalog # AF528

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCL24/Eotaxin-2/MPIF-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
Ile24-Val119
Accession # Q9JKC0

Specificity

Detects CCL24/Eotaxin‑2/MPIF‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Eotaxin-2 is observed, and less than 1% cross-reactivity with recombinant mouse Eotaxin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Mouse CCL24/Eotaxin‑2 Antibody.

Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Mouse CCL24/Eotaxin‑2 Antibody.

Recombinant Mouse CCL24/Eotaxin-2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin-2 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL24/ Eotaxin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF528). The ND50 is typically 0.5-2.5 µg/mL.
Mouse CCL24 / Eotaxin-2 / MPIF-2 ELISA Standard Curve

Mouse CCL24 / Eotaxin-2 / MPIF-2 ELISA Standard Curve

Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2 aa 24-119 (Catalog # 528-MP) was serially diluted and captured by Rat Anti-Mouse CCL24/Eotaxin‑2/MPIF‑2 Monoclonal Antibody (Catalog # MAB528) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL24/Eotaxin‑2/MPIF‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF528) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 528-MP)

Neutralization

Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.02 µg/mL Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin.

Alternate Names

Eotaxin-2, MPIF-2, MPIF2

Entrez Gene IDs

6369 (Human); 56221 (Mouse)

Gene Symbol

CCL24

UniProt

Q9JKC0

Additional CCL24/Eotaxin-2/MPIF-2 Products

Product Documents for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

For research use only

Citations for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

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