Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects CCL24/Eotaxin‑2/MPIF‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Eotaxin-2 is observed, and less than 1% cross-reactivity with recombinant mouse Eotaxin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
    Ile24-Val119
    Accession # Q9JKC0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 528-MP)
  • Neutralization
    Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.02 µg/mL Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Mouse CCL24/Eotaxin‑2 Antibody. Recombinant Mouse CCL24/Eotaxin‑2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin‑2 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL24/
Eotaxin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF528). The ND50 is typically 0.5-2.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin.

  • Entrez Gene IDs:
    6369 (Human); 56221 (Mouse)
  • Alternate Names:
    CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Species
Applications
Sample Type
  1. TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
    Authors: Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson
    PLoS Pathog., 2017;13(7):e1006536.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  3. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. CCR3 is a target for age-related macular degeneration diagnosis and therapy.
    Authors: Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, Kaneko H, Albuquerque RJ, Dridi S, Saito K, Raisler BJ, Budd SJ, Geisen P, Munitz A, Ambati BK, Green MG, Ishibashi T, Wright JD, Humbles AA, Gerard CJ, Ogura Y, Pan Y, Smith JR, Grisanti S, Hartnett ME, Rothenberg ME, Ambati J
    Nature, 2009;460(7252):225-30.
    Species: Mouse
    Sample Type: Vitreous Humor
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse CCL24/Eotaxin-2/MPIF-2 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information