Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY528
Ancillary Products Available
Mouse CCL24 / Eotaxin-2 / MPIF-2 ELISA Standard Curve
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Product Details
Procedure
Citations (30)
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Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CCL24/Eotaxin-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

Mouse CCL24 / Eotaxin-2 / MPIF-2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor 2 (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. Mouse Eotaxin-2 precursor protein shares approximately 58% amino acid sequence identity with human Eotaxin-2.

Entrez Gene IDs:
6369 (Human); 56221 (Mouse)
Alternate Names:
CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Fucoxanthin Ameliorates Oxidative Stress and Airway Inflammation in Tracheal Epithelial Cells and Asthmatic Mice
    Authors: SJ Wu, CJ Liou, YL Chen, SC Cheng, WC Huang
    Cells, 2021;10(6):.
    Species: Mouse
    Sample Types: BALF
  2. IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M
    Authors: F Botelho, A Dubey, EA Ayaub, R Park, A Yip, A Humbles, R Kolbeck, CD Richards
    Mediators of Inflammation, 2020;2020(0):4087315.
    Species: Mouse
    Sample Types: BALF
  3. Allergic inflammation is initiated by IL-33-dependent crosstalk between mast cells and basophils
    Authors: CL Hsu, KD Chhiba, R Krier-Burr, S Hosakoppal, S Berdnikovs, ML Miller, PJ Bryce
    PLoS ONE, 2020;15(1):e0226701.
    Species: Mouse
    Sample Types: Cell Culture Supern
  4. The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model
    Authors: IS Park, JH Kim, JS Bae, DK Kim, JH Mo
    Mediators Inflamm., 2020;2020(0):6982438.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. House Dust Mite Induces Bone Marrow IL-33-Responsive ILC2s and TH Cells
    Authors: E Boberg, K Johansson, C Malmhäll, J Weidner, M Rådinger
    Int J Mol Sci, 2020;21(11):.
    Species: Mouse
    Sample Types: BALF
  6. Glucagon reduces airway hyperreactivity, inflammation, and remodeling induced by ovalbumin
    Authors: DBR Insuela, CT Azevedo, DS Coutinho, NS Magalhães, MR Ferrero, TPT Ferreira, CM Cascabulho, A Henriques-, PC Olsen, BL Diaz, PMR Silva, RSB Cordeiro, MA Martins, VF Carvalho
    Sci Rep, 2019;9(1):6478.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Protective Effects of Licochalcone A Improve Airway Hyper-Responsiveness and Oxidative Stress in a Mouse Model of Asthma
    Authors: WC Huang, CY Liu, SC Shen, LC Chen, KW Yeh, SH Liu, CJ Liou
    Cells, 2019;8(6):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Eosinophil-derived CCL-6 impairs hematopoietic stem cell homeostasis
    Authors: C Zhang, W Yi, F Li, X Du, H Wang, P Wu, C Peng, M Luo, W Hua, CC Wong, JJ Lee, W Li, Z Chen, S Ying, Z Ju, H Shen
    Cell Res., 2018;0(0):.
    Species: Mouse
    Sample Types: BALF
  9. Androgen and Androgen Receptor as Enhancers of M2 Macrophage Polarization in Allergic Lung Inflammation
    Authors: M Becerra-Dí, AB Strickland, A Keselman, NM Heller
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: BALF
  10. Eosinophils support adipocyte maturation and promote glucose tolerance in obesity
    Authors: EH Lee, M Itan, J Jang, HJ Gu, P Rozenberg, MK Mingler, T Wen, J Yoon, SY Park, JY Roh, CS Choi, WJ Park, A Munitz, Y Jung
    Sci Rep, 2018;8(1):9894.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Toll-Interacting Protein, Tollip, Inhibits IL-13-Mediated Pulmonary Eosinophilic Inflammation in Mice
    Authors: Y Ito, N Schaefer, A Sanchez, D Francisco, R Alam, RJ Martin, JG Ledford, C Stevenson, D Jiang, L Li, M Kraft, HW Chu
    J Innate Immun, 2018;0(0):.
    Species: Mouse
    Sample Types: BALF
  12. Knob protein enhances epithelial barrier integrity and attenuates airway inflammation
    Authors: SG Ha, M Dileepan, XN Ge, BN Kang, YG Greenberg, A Rao, G Muralidhar, L Medina-Kau, MA Thompson, CM Pabelick, SM O'Grady, SP Rao, P Sriramarao
    J. Allergy Clin. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Capture
  13. Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
    Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
    Cell Death Dis, 2017;8(4):e2735.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Regulatory effects of Interleukin (IL)-15 on allergen-induced airway obstruction
    Authors: SU Venkatesha, X Zhu, P Rajavelu, R Niranjan, M Manohar, AK Verma, JA Lasky, A Mishra
    J. Allergy Clin. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: BALF
  15. Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Types: BALF
  16. IL-1R1-MyD88 axis elicits papain-induced lung inflammation
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
    Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
    Nat Commun, 2015;6(0):6224.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. B cells play key roles in th2-type airway immune responses in mice exposed to natural airborne allergens.
    Authors: Drake L, Iijima K, Hara K, Kobayashi T, Kephart G, Kita H
    PLoS ONE, 2015;10(3):e0121660.
    Species: Mouse
    Sample Types: BALF
  19. Mycoplasma pneumoniae CARDS toxin exacerbates ovalbumin-induced asthma-like inflammation in BALB/c mice.
    Authors: Medina, Jorge L, Coalson, Jacqueli, Brooks, Edward G, Le Saux, Claude J, Winter, Vicki T, Chaparro, Adriana, Principe, Molly F, Solis, Laura, Kannan, T R, Baseman, Joel B, Dube, Peter H
    PLoS ONE, 2014;9(7):e102613.
    Species: Mouse
    Sample Types: BALF
  20. IRF-3, IRF-7, and IPS-1 promote host defense against acute human metapneumovirus infection in neonatal mice.
    Authors: Spann K, Loh Z, Lynch J, Ullah A, Zhang V, Baturcam E, Werder R, Khajornjiraphan N, Rudd P, Loo Y, Suhrbier A, Gale M, Upham J, Phipps S
    Am J Pathol, 2014;184(6):1795-806.
    Species: Mouse
    Sample Types: Cell Lysates
  21. MicroRNA-155 is essential for T(H)2-mediated allergen-induced eosinophilic inflammation in the lung.
    Authors: Malmhall C, Alawieh S, Lu Y, Sjostrand M, Bossios A, Eldh M, Radinger M
    J Allergy Clin Immunol, 2014;133(5):1429-38, 1438.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. Mice deficient in the St3gal3 gene product alpha2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation.
    Authors: Kiwamoto T, Brummet M, Wu F, Motari M, Smith D, Schnaar R, Zhu Z, Bochner B
    J Allergy Clin Immunol, 2014;133(1):240-7.e1-3.
    Species: Mouse
    Sample Types: BALF
  23. Peptidoglycan recognition protein 1 enhances experimental asthma by promoting Th2 and Th17 and limiting regulatory T cell and plasmacytoid dendritic cell responses.
    Authors: Park S, Jing X, Gupta D, Dziarski R
    J Immunol, 2013;190(7):3480-92.
    Species: Mouse
    Sample Types: Tissue Homogenates
  24. Eosinophil viability is increased by acidic pH in a cAMP- and GPR65-dependent manner.
    Authors: Kottyan LC, Collier AR, Cao KH, Niese KA, Hedgebeth M, Radu CG, Witte ON, Khurana Hershey GK, Rothenberg ME, Zimmermann N
    Blood, 2009;114(13):2774-82.
    Species: Mouse
    Sample Types: Cell Lysates
  25. CD11b+ myeloid cells are the key mediators of Th2 cell homing into the airway in allergic inflammation.
    Authors: Medoff BD, Seung E, Hong S, Thomas SY, Sandall BP, Duffield JS, Kuperman DA, Erle DJ, Luster AD
    J. Immunol., 2009;182(1):623-35.
    Species: Mouse
    Sample Types: BALF
  26. Surfactant protein D alters allergic lung responses in mice and human subjects.
    Authors: Brandt EB, Mingler MK, Stevenson MD, Wang N, Khurana Hershey GK, Whitsett JA, Rothenberg ME
    J. Allergy Clin. Immunol., 2008;121(5):1140-1147.e2.
    Species: Mouse
    Sample Types: BALF
  27. Distinct roles for IL-13 and IL-4 via IL-13 receptor alpha1 and the type II IL-4 receptor in asthma pathogenesis.
    Authors: Munitz A, Brandt EB, Mingler M, Finkelman FD, Rothenberg ME
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7240-5.
    Species: Mouse
    Sample Types: BALF
  28. Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma.
    Authors: Ochkur SI, Jacobsen EA, Protheroe CA, Biechele TL, Pero RS, McGarry MP, Wang H, O'Neill KR, O'Neill KR, Colbert DC, Colby TV, Shen H, Blackburn MR, Irvin CC, Lee JJ, Lee NA
    J. Immunol., 2007;178(12):7879-89.
    Species: Mouse
    Sample Types: BALF
  29. Regulation of allergen-induced bone marrow eosinophilopoiesis: role of CD4+ and CD8+ T cells.
    Authors: Radinger M, Bossios A, Alm AS, Jeurink P, Lu Y, Malmhall C, Sjostrand M, Lotvall J
    Allergy, 2007;62(12):1410-8.
    Species: Mouse
    Sample Types: BALF
  30. Persistent effects induced by IL-13 in the lung.
    Authors: Fulkerson PC, Fischetti CA, Hassman LM, Nikolaidis NM, Rothenberg ME
    Am. J. Respir. Cell Mol. Biol., 2006;35(3):337-46.
    Species: Mouse
    Sample Types: BALF

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Reviews for Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA

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Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA
By Anonymous on 09/13/2019
Sample Tested: Serum

Elevated expression in mouse serum upon IL-13 stimulation


Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA
By Murli Manohar on 08/25/2016
Sample Tested: Pancreas