Detects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/MIP‑1 beta is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed. Neutralizes the biological activity of recombinant mouse CCL3 and will not block the biological activity of recombinant human CCL3.
Monoclonal Rat IgG2A Clone # 39624
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse CCL3/MIP-1 alpha Ala24-Ala92 Accession # P10855
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Mouse CCL3/MIP-1 alpha Isoform LD78a (Catalog # 450-MA) under non-reducing conditions only
Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in human monocytes. The Neutralization Dose (ND50) is typically 5-25 µg/mL in the presence of 100 ng/mL Recombinant Mouse CCL3/MIP‑1 alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Mouse CCL3/ MIP‑1 alpha Antibody.
Recombinant Mouse CCL3/MIP‑1 alpha (Catalog # 450‑MA) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by Hema3 Staining System (Fisher Scientific). Chemotaxis elicited by Recombinant Mouse CCL3/MIP‑1 alpha (100 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB450). The ND50 is typically 5-25 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha
The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta (C‑C) subfamily of chemokines.
Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8+ and CD4+ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.
Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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