Mouse CCL3/MIP-1 alpha Antibody

(9 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/
    MIP‑1 beta  is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 39624
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CCL3/MIP-1 alpha
    Ala24-Ala92
    Accession # P10855
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CCL3/MIP-1 alpha Isoform LD78a (Catalog # 450-MA) under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha

The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta  (C‑C) subfamily of chemokines.

Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8+ and CD4+ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.

  • References:
    1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  • Entrez Gene IDs:
    6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine)
  • Alternate Names:
    C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Mechanisms of Invariant NKT Cell Activity in Restraining Bacillus anthracis Systemic Dissemination
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. Immune signatures of protective spleen memory CD8 T cells
    Sci Rep, 2016;6(0):37651.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma.
    Authors: Deepak P, Kumar S, Kishore D, Acharya A
    Int. Immunol., 2010;22(1):53-63.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  5. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007;245(2):70-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  6. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Yang L, Mosmann T
    Eur. J. Immunol., 2004;34(6):1617-26.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  7. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Mosmann T
    Eur. J. Immunol., 2004;34(6):1617-26.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  8. A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
    Authors: Reading PC, Symons JA, Smith GL
    J. Immunol., 2003;170(3):1435-42.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  9. Regulatory effects of eotaxin on acute lung inflammatory injury.
    Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA
    J. Immunol., 2001;166(8):5208-18.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2A Isotype Control

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WB, Simple Western HAF005 7  
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WB BAF005 2
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Goat Anti-Rat IgG Antibody

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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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