Mouse CCL6/C10 Antibody

Catalog # Availability Size / Price Qty
MAB4872-100
MAB4872-SP
Mouse CCL6/C10 Antibody in ELISA
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Mouse CCL6/C10 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL6/C10 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 262008
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CCL6/C10
Gly22-Ala116
Accession # P27784
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB4871).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse CCL6/C10 DuoSet ELISA Kit (Catalog # DY487) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Mouse CCL6/C10 ELISA Standard Curve. Recombinant Mouse CCL6/C10 protein was serially diluted 2-fold and captured by Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB4871) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB4872) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL6/C10

Mouse CCL6, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11.

Entrez Gene IDs
20305 (Mouse); 287910 (Rat)
Alternate Names
C10; CCL6; MRP-1; SCYA6

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