Mouse CCL6/C10 Antibody Summary
Accession # P27784
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB4871).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse CCL6/C10 DuoSet ELISA Kit (Catalog # DY487) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Mouse CCL6/C10 ELISA Standard Curve. Recombinant Mouse CCL6/C10 protein was serially diluted 2-fold and captured by Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB4871) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB4872) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse CCL6, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11.
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