Detection of CCR2 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G APC‑conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rat Anti-Mouse/Rat CCR2 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB5538T) or (B) Rat IgG2B Alexa Fluor 594 Isotype Control (Catalog # IC013T). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR2 in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP by Flow Cytometry.
NS0 mouse myeloma cell line transfected with mouse CCR2 and eGFP was stained with either (A) Rat Anti-Mouse/Rat CCR2 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB5538T) or (B) Rat IgG2B Alexa Fluor 594 Isotype Control (Catalog # IC013T). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that binds CCL2, CCL7, CCL8, CCL12 (in mouse only) and CCL13. CCR2 is expressed by multiple hematopoietic cells plus, endothelial cells, fibroblasts, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.
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