Mouse CCR2 Fluorescein-conjugated Antibody

(8 citations)
(3 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.
  • Source
    Monoclonal Rat IgG2B Clone # 475301
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    L1.2 mouse pro-B cell line transfected with mouse CCR2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR2 in Mouse Splenocytes byFlow Cytometry. Mouse splenocytes were stained with RatAnti-Mouse CCR2 Fluorescein‑conjugated Monoclonal Antibody (Catalog# FAB5538F) and Rat Anti-Mouse Gr‑1/Ly‑6GPE‑conjugated Monoclonal Antibody (Catalog # FAB1037P).View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR2
CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.
  • Entrez Gene IDs:
    729230 (Human); 12772 (Mouse); 60463 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Small molecule-based inhibition of MEK1/2 proteins dampens inflammatory responses to malaria, reduces parasite load, and mitigates pathogenic outcomes
    Authors: X Wu, KK Dayanand, RP Thylur, CC Norbury, DC Gowda
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Innate Allorecognition Results in Rapid Accumulation of Monocyte-Derived Dendritic Cells
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Monocyte-Derived Macrophages Contribute to Spontaneous Long-Term Functional Recovery after Stroke in Mice
    Authors: S Wattananit, D Tornero, N Graubardt, T Memanishvi, E Monni, J Tatarishvi, G Miskinyte, R Ge, H Ahlenius, O Lindvall, M Schwartz, Z Kokaia
    J Neurosci, 2016;36(15):4182-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Combined effect of insulin-like growth factor-1 and CC chemokine ligand 2 on angiogenic events in endothelial cells.
    Authors: Viana I, de Almeida M, Lins M, dos Santos Reis M, de Araujo Vieira L, Smaniotto S
    PLoS ONE, 2015;10(4):e0121249.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
    Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
    Nature, 2015;515(7525):130-3.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Differential TGF-beta Signaling in Glial Subsets Underlies IL-6-Mediated Epileptogenesis in Mice.
    Authors: Levy N, Milikovsky D, Baranauskas G, Vinogradov E, David Y, Ketzef M, Abutbul S, Weissberg I, Kamintsky L, Fleidervish I, Friedman A, Monsonego A
    J Immunol, 2015;195(4):1713-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Myeloid-derived suppressor cells contribute to Staphylococcus aureus orthopedic biofilm infection.
    Authors: Heim, Cortney, Vidlak, Debbie, Scherr, Tyler D, Kozel, Jessica, Holzapfel, Melissa, Muirhead, David E, Kielian, Tammy
    J Immunol, 2014;192(8):3778-92.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. Toxoplasma gondii profilin promotes recruitment of Ly6Chi CCR2+ inflammatory monocytes that can confer resistance to bacterial infection.
    Authors: Neal L, Knoll L
    PLoS Pathog, 2014;10(6):e1004203.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Fluorescein-conjugated Antibody

Ctrl IC013F 3  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 3 reviews)

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Read what people are saying who have used Mouse CCR2 Fluorescein-conjugated Antibody.

We have 3 reviews tested in 1 species: Mouse.
We have 3 review tested in 1 application: Flow Cytometry.

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Very Good
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Mouse
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Images Ratings Applications Species Reviewed By Date Details
  Very Good
 Flow Mouse Anonymous 08/17/2016
Show

Summary

ApplicationFlow Cytometry
Sample TestedTumor cell lyastes
SpeciesMouse
Flow Cytometry Mouse CCR2 Fluorescein‑conjugated Antibody FAB5538F
Enlarge
Very Good
 Flow Mouse Anonymous 10/26/2015
Show
Image Details
Flow Cytometry Mouse CCR2 Fluorescein‑conjugated Antibody FAB5538F
Flow Cytometry: Mouse CCR2 Fluorescein‑conjugated Antibody [FAB5538F]

Summary

ApplicationFlow Cytometry
Sample TestedSplenocytes
SpeciesMouse

Other Experimental Details

Other Experimental DetailsImage is gated on CD19- CD3- GR1- splenocytes.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: HBSS w/ 2% FBS
Dilution: 1:20
Flow Cytometry Mouse CCR2 Fluorescein‑conjugated Antibody FAB5538F
Enlarge
Very Good
 Flow Mouse Anonymous 10/26/2015
Show
Image Details
Flow Cytometry Mouse CCR2 Fluorescein‑conjugated Antibody FAB5538F
Flow Cytometry: Mouse CCR2 Fluorescein‑conjugated Antibody [FAB5538F]

Summary

ApplicationFlow Cytometry
Sample TestedMurine mesenteric lymph node
SpeciesMouse

Other Experimental Details

Other Experimental DetailsGated on non-B non-T cells.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: HBSS w/ 2% FBS
Dilution: 1:20

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