Mouse CCR2 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5538F-025
FAB5538F-100

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Detection of CCR2 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (5)

Mouse CCR2 Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 475301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
L1.2 mouse pro-B cell line transfected with mouse CCR2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR2 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR2 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB5538F) and Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (Catalog # FAB1037P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Entrez Gene IDs
729230 (Human); 12772 (Mouse); 60463 (Rat)
Alternate Names
C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

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Citations for Mouse CCR2 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Meningeal lymphatics affect microglia responses and anti-A&beta immunotherapy
    Authors: S Da Mesquit, Z Papadopoul, T Dykstra, L Brase, FG Farias, M Wall, H Jiang, CD Kodira, KA de Lima, J Herz, A Louveau, DH Goldman, AF Salvador, S Onengut-Gu, E Farber, N Dabhi, T Kennedy, MG Milam, W Baker, I Smirnov, SS Rich, Dominantly, BA Benitez, CM Karch, RJ Perrin, M Farlow, JP Chhatwal, DM Holtzman, C Cruchaga, O Harari, J Kipnis
    Nature, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Aging-associated deficit in CCR7 is linked to worsened glymphatic function, cognition, neuroinflammation, and &beta-amyloid pathology
    Authors: S Da Mesquit, J Herz, M Wall, T Dykstra, KA de Lima, GT Norris, N Dabhi, T Kennedy, W Baker, J Kipnis
    Science Advances, 2021;7(21):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Attenuated Macrophage Infiltration in Glomeruli of Aged Mice Resulting in Ameliorated Kidney Injury in Nephrotoxic Serum Nephritis
    Authors: Y Kaneko, T Cho, Y Sato, K Goto, S Yamamoto, S Goto, MP Madaio, I Narita
    J. Gerontol. A Biol. Sci. Med. Sci., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Myeloid p38? signaling promotes intestinal IGF-1 production and inflammation-associated tumorigenesis
    Authors: C Youssif, M Cubillos-R, M Comalada, E Llonch, C Perna, N Djouder, AR Nebreda
    EMBO Mol Med, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. BCG Educates Hematopoietic Stem Cells to Generate Protective Innate Immunity against Tuberculosis
    Authors: E Kaufmann, J Sanz, JL Dunn, N Khan, LE Mendonça, A Pacis, F Tzelepis, E Pernet, A Dumaine, JC Grenier, F Mailhot-Lé, E Ahmed, J Belle, R Besla, B Mazer, IL King, A Nijnik, CS Robbins, LB Barreiro, M Divangahi
    Cell, 2018;172(1):176-190.e19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Small molecule-based inhibition of MEK1/2 proteins dampens inflammatory responses to malaria, reduces parasite load, and mitigates pathogenic outcomes
    Authors: X Wu, KK Dayanand, RP Thylur, CC Norbury, DC Gowda
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Innate Allorecognition Results in Rapid Accumulation of Monocyte-Derived Dendritic Cells
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Monocyte-Derived Macrophages Contribute to Spontaneous Long-Term Functional Recovery after Stroke in Mice
    Authors: S Wattananit, D Tornero, N Graubardt, T Memanishvi, E Monni, J Tatarishvi, G Miskinyte, R Ge, H Ahlenius, O Lindvall, M Schwartz, Z Kokaia
    J Neurosci, 2016;36(15):4182-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Combined effect of insulin-like growth factor-1 and CC chemokine ligand 2 on angiogenic events in endothelial cells.
    Authors: Viana I, de Almeida M, Lins M, dos Santos Reis M, de Araujo Vieira L, Smaniotto S
    PLoS ONE, 2015;10(4):e0121249.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
    Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
    Nature, 2015;515(7525):130-3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Differential TGF-beta Signaling in Glial Subsets Underlies IL-6-Mediated Epileptogenesis in Mice.
    Authors: Levy N, Milikovsky D, Baranauskas G, Vinogradov E, David Y, Ketzef M, Abutbul S, Weissberg I, Kamintsky L, Fleidervish I, Friedman A, Monsonego A
    J Immunol, 2015;195(4):1713-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Myeloid-derived suppressor cells contribute to Staphylococcus aureus orthopedic biofilm infection.
    Authors: Heim, Cortney, Vidlak, Debbie, Scherr, Tyler D, Kozel, Jessica, Holzapfel, Melissa, Muirhead, David E, Kielian, Tammy
    J Immunol, 2014;192(8):3778-92.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Toxoplasma gondii profilin promotes recruitment of Ly6Chi CCR2+ inflammatory monocytes that can confer resistance to bacterial infection.
    Authors: Neal L, Knoll L
    PLoS Pathog, 2014;10(6):e1004203.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Central Role of IL-23 and IL-17 Producing Eosinophils as Immunomodulatory Effector Cells in Acute Pulmonary Aspergillosis and Allergic Asthma.
    Authors: Guerra E, Lee C, Specht C, Yadav B, Huang H, Akalin A, Huh J, Mueller C, Levitz S
    PLoS Pathog, 0;13(1):e1006175.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse CCR2 Fluorescein-conjugated Antibody

Average Rating: 4.4 (Based on 5 Reviews)

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Mouse CCR2 Fluorescein-conjugated Antibody
By William Jackson on 11/12/2018
Application: Flow Sample Tested: Mouse spleenocytes Species: Mouse

Mouse CCR2 Fluorescein-conjugated Antibody
By Anonymous on 11/01/2018
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Mouse

Mouse CCR2 Fluorescein-conjugated Antibody
By Anonymous on 08/17/2016
Application: Flow Sample Tested: Tumor cell lyastes Species: Mouse

Mouse CCR2 Fluorescein‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Splenocytes Species: Mouse

Image is gated on CD19- CD3- GR1- splenocytes.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: HBSS w/ 2% FBS
Dilution: 1:20


Mouse CCR2 Fluorescein‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Murine mesenteric lymph node Species: Mouse

Gated on non-B non-T cells.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: HBSS w/ 2% FBS
Dilution: 1:20