Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 475301
View all CCR2 Primary Antibodies »

Product Specifications

Immunogen

L1.2 mouse pro-B cell line transfected with mouse CCR2

Specificity

Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CCR2 Fluorescein‑conjugated Antibody

Detection of CCR2 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CCR2 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CCR2 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB5538F) and Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (Catalog # FAB1037P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CCR2 Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Reviewed Applications

Read 5 reviews rated 4.4 using FAB5538F in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Gene Symbol

CCR2

Additional CCR2 Products

Product Documents for Mouse CCR2 Fluorescein‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCR2 Fluorescein‑conjugated Antibody

For research use only

Citations for Mouse CCR2 Fluorescein‑conjugated Antibody

Customer Reviews for Mouse CCR2 Fluorescein‑conjugated Antibody (5)

4.4 out of 5
5 Customer Ratings
5 Stars
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4 Stars
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1 Stars
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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Mouse CCR2 Fluorescein-conjugated Antibody
    Name: William Jackson
    Application: Flow Cytometry
    Sample Tested: Mouse spleenocytes
    Species: Mouse
    Verified Customer | Posted 11/12/2018
    Mouse CCR2 Fluorescein‑conjugated Antibody FAB5538F
  • Mouse CCR2 Fluorescein-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Mouse
    Verified Customer | Posted 11/01/2018
    Mouse CCR2 Fluorescein‑conjugated Antibody FAB5538F
  • Mouse CCR2 Fluorescein-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Tumor cell lyastes
    Species: Mouse
    Verified Customer | Posted 08/17/2016
  • Mouse CCR2 Fluorescein‑conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Murine mesenteric lymph node
    Species: Mouse
    Verified Customer | Posted 10/26/2015
    Gated on non-B non-T cells. <br />Specificity: Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: HBSS w/ 2% FBS<br />Dilution: 1:20
    Mouse CCR2 Fluorescein‑conjugated Antibody FAB5538F
  • Mouse CCR2 Fluorescein‑conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Splenocytes
    Species: Mouse
    Verified Customer | Posted 10/26/2015
    Image is gated on CD19- CD3- GR1- splenocytes. <br />Specificity: Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: HBSS w/ 2% FBS<br />Dilution: 1:20
    Mouse CCR2 Fluorescein‑conjugated Antibody FAB5538F

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