Mouse CCR2 PE-conjugated Antibody

(23 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.
  • Source
    Monoclonal Rat IgG2B Clone # 475301
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    L1.2 mouse pro-B cell line transfected with mouse CCR2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR2 in Mouse Splenocytes byFlow Cytometry. Mouse splenocytes were stained with RatAnti-Mouse CCR2 PE‑conjugated Monoclonal Antibody (Catalog #FAB5538P) and Rat Anti-Mouse Gr‑1/Ly‑6GFluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F).Quadrant marker was set based on isotype control antibody staining (Catalog # IC013P). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR2
CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that binds CCL2, CCL7, CCL8, CCL12 (in mouse only) and CCL13. CCR2 is expressed by multiple hematopoietic cells plus, endothelial cells, fibroblasts, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.
  • Entrez Gene IDs:
    729230 (Human); 12772 (Mouse); 60463 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Delayed decompression exacerbates ischemia-reperfusion injury in cervical compressive myelopathy
    Authors: PM Vidal, SK Karadimas, A Ulndreaj, AM Laliberte, L Tetreault, S Forner, J Wang, WD Foltz, MG Fehlings
    JCI Insight, 2017;2(11):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Recruitment of neutrophils mediated by V?2 ?? T cells deteriorate liver fibrosis induced by Schistosoma japonicum infection in C57BL/6 mice
    Authors: L Zheng, Y Hu, Y Wang, X Huang, Y Xu, Y Shen, J Cao
    Infect. Immun., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Distinct functions of CXCR4, CCR2, and CX3CR1 direct dendritic cell precursors from the bone marrow to the lung
    Authors: H Nakano, MR Lyons-Cohe, GS Whitehead, K Nakano, DN Cook
    J. Leukoc. Biol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. The Yin/Yan of CCL2: a minor role in neutrophil anti-tumor activity in vitro but a major role on the outgrowth of metastatic breast cancer lesions in the lung in vivo
    Authors: N Lavender, J Yang, SC Chen, J Sai, CA Johnson, P Owens, GD Ayers, A Richmond
    BMC Cancer, 2017;17(1):88.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. AMPK-Activated Protein Kinase Suppresses Ccr2 Expression by Inhibiting the NF-kappaB Pathway in RAW264.7 Macrophages.
    Authors: Kumase F, Takeuchi K, Morizane Y, Suzuki J, Matsumoto H, Kataoka K, Al-Moujahed A, Maidana D, Miller J, Vavvas D
    PLoS ONE, 2016;11(1):e0147279.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Alternatively activated macrophages determine repair of the infarcted adult murine heart
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Tumor necrosis factor drives increased splenic monopoiesis in old mice
    Authors: D Loukov, A Naidoo, A Puchta, JL Marin, DM Bowdish
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. Treatment with cestode parasite antigens recruits CCR2+ myeloid cells, the adoptive transfer of which ameliorates colitis
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. MKEY, a Peptide Inhibitor of CXCL4-CCL5 Heterodimer Formation, Protects Against Stroke in Mice
    J Am Heart Assoc, 2016;5(9):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  11. Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced anti-viral therapeutic effects
    Sci Rep, 2016;6(0):39071.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  12. Batf3-dependent CD103+ dendritic cells are major producers of IL-12 that drive local Th1 immunity against Leishmania major infection in mice.
    Authors: Martinez-Lopez M, Iborra S, Conde-Garrosa R, Sancho D
    Eur J Immunol, 2015;45(1):119-29.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  13. IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.
    Authors: Coffelt S, Kersten K, Doornebal C, Weiden J, Vrijland K, Hau C, Verstegen N, Ciampricotti M, Hawinkels L, Jonkers J, de Visser K
    Nature, 2015;522(7556):345-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  14. Male bovine GH transgenic mice have decreased adiposity with an adipose depot-specific increase in immune cell populations.
    Authors: Benencia, Fabian, Harshman, Stephani, Duran-Ortiz, Silvana, Lubbers, Ellen R, List, Edward O, Householder, Lara, Al-Naeeli, Mawadda, Liang, Xiaoyu, Welch, Lonnie, Kopchick, John J, Berryman, Darlene
    Endocrinology, 2015;156(5):1794-803.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  15. Development of a pHrodo-based assay for the assessment of in vitro and in vivo erythrophagocytosis during experimental trypanosomosis.
    Authors: Stijlemans, Benoit, Cnops, Jennifer, Naniima, Peter, Vaast, Axel, Bockstal, Viki, De Baetselier, Patrick, Magez, Stefan
    PLoS Negl Trop Dis, 2015;9(3):e0003561.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  16. Dynamics and Transcriptomics of Skin Dendritic Cells and Macrophages in an Imiquimod-Induced, Biphasic Mouse Model of Psoriasis.
    Authors: Terhorst D, Chelbi R, Wohn C, Malosse C, Tamoutounour S, Jorquera A, Bajenoff M, Dalod M, Malissen B, Henri S
    J Immunol, 2015;195(10):4953-61.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  17. CD11b is protective in complement-mediated immune complex glomerulonephritis.
    Authors: Alexander J, Chaves L, Chang A, Jacob A, Ritchie M, Quigg R
    Kidney Int, 2015;87(5):930-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  18. Loss of CD11b exacerbates murine complement-mediated tubulointerstitial nephritis.
    Authors: Chaves, Lee Dani, Bao, Lihua, Wang, Ying, Chang, Anthony, Haas, Mark, Quigg, Richard
    PLoS ONE, 2014;9(3):e92051.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  19. Molecular and cellular neuroinflammatory status of mouse brain after systemic lipopolysaccharide challenge: importance of CCR2/CCL2 signaling.
    Authors: Cazareth J, Guyon A, Heurteaux C, Chabry J, Petit-Paitel A
    J Neuroinflammation, 2014;11(0):132.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  20. Obesity alters adipose tissue macrophage iron content and tissue iron distribution.
    Authors: Orr J, Kennedy A, Anderson-Baucum E, Webb C, Fordahl S, Erikson K, Zhang Y, Etzerodt A, Moestrup S, Hasty A
    Diabetes, 2014;63(2):421-32.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  21. The monocyte to macrophage transition in the murine sterile wound.
    Authors: Crane, Meredith, Daley, Jean M, van Houtte, Olivier, Brancato, Samielle, Henry, William, Albina, Jorge E
    PLoS ONE, 2014;9(1):e86660.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  22. Monocyte chemoattractant protein-1/CCL2 produced by stromal cells promotes lung metastasis of 4T1 murine breast cancer cells.
    Authors: Yoshimura T, Howard O, Ito T, Kuwabara M, Matsukawa A, Chen K, Liu Y, Liu M, Oppenheim J, Wang J
    PLoS ONE, 2013;8(3):e58791.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  23. Chemokine receptor CX3CR1 contributes to macrophage survival in tumor metastasis.
    Authors: Zheng, Jiao, Yang, Min, Shao, Jianghua, Miao, Yanju, Han, Jiahuai, Du, Jie
    Mol Cancer, 2013;12(1):141.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 23 Citations
Isotype Controls
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Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 1 review)

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Summary

ApplicationFlow Cytometry
Sample TestedTumor cell lyastes
SpeciesMouse

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