Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Mass Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 475301
View all CCR2 Primary Antibodies »

Product Specifications

Immunogen

L1.2 mouse pro-B cell line transfected with mouse CCR2

Specificity

Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CCR2 PE‑conjugated Antibody

Detection of CCR2 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CCR2 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CCR2 PE-conjugated Monoclonal Antibody (Catalog # FAB5538P) and Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F). Quadrant marker was set based on isotype control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse CCR2 by Flow Cytometry

Detection of Mouse CCR2 by Flow Cytometry

Depletion of CCR2 cells enhances tumor radiosensitivity. a Scheme of treatment. b Treated tumor was measured every 3-4 days for 33 days starting from the day of radiation (n = 10). c Percentage of mice still bearing tumor at the end of the treatments (n = 10), P < 0.05. d Flow cytometry profile of CCR2+Ly6Chi in tumors 3 days post the start of treatments. Left, representative flow graph, gated on CD45+CD11b+cells; Middle, percentage of CD45+; Right, absolute number of cells (n = 4). NS, non-significant.*P < 0.05; **P < 0.01; ***P < 0.001. Each experiment was repeated 3 times. Data are presented as mean ± s.e.m Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29170400), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CCR2 by Flow Cytometry

Detection of Mouse CCR2 by Flow Cytometry

MDSC depletion enhances tumor response to radiation and STING agonist cGAMP treatment. a Flow cytometry analysis of immune CCR2+Ly6chi cells 3 days after IR (n = 3). b Tumor growth curves; n = 6; c percentage of tumor bearing mice ending on 33 days after starting of the treatments. d, e ratios of percentage of CD8 over CCR2+Ly6chi and Treg cells in treated tumors, respectively. n = 3. *P < 0.05; **P < 0.01. The experiments were repeated three times. Data are presented as mean ± s.e.m Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29170400), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCR2 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Reviewed Applications

Read 4 reviews rated 4.3 using FAB5538P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that binds CCL2, CCL7, CCL8, CCL12 (in mouse only) and CCL13. CCR2 is expressed by multiple hematopoietic cells plus, endothelial cells, fibroblasts, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Gene Symbol

CCR2

Additional CCR2 Products

Product Documents for Mouse CCR2 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCR2 PE‑conjugated Antibody

For research use only

Citations for Mouse CCR2 PE‑conjugated Antibody

Customer Reviews for Mouse CCR2 PE‑conjugated Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
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1 Stars
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Showing  1 - 4 of 4 reviews Showing All
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  • Mouse CCR2 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Adult pancreas and Pancreas tissue
    Species: Mouse
    Verified Customer | Posted 05/03/2022
    Works well on pancreatic myeloid cells, but unfortunately requires a 1:50 dilution.
    Mouse CCR2 PE‑conjugated Antibody FAB5538P
  • Mouse CCR2 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 04/01/2020
    Mouse CCR2 PE‑conjugated Antibody FAB5538P
  • Mouse CCR2 PE-conjugated Antibody
    Name: Christian Ostalecki
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Lymph node tissue
    Species: Mouse
    Verified Customer | Posted 06/06/2018
    Mouse CCR2 PE‑conjugated Antibody FAB5538P
  • Mouse CCR2 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Tumor cell lyastes
    Species: Mouse
    Verified Customer | Posted 08/24/2016

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