Mouse CCR2 Alexa Fluor® 700-conjugated Antibody

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Detection of CCR2 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (11)
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Reviews (1)

Mouse CCR2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Detects mouse and rat CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.
Monoclonal Rat IgG2B Clone # 475301
Protein A or G purified from hybridoma culture supernatant
L1.2 mouse pro-B cell line transfected with mouse CCR2
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)


Recommended Concentration
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR2 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR2 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB5538N) and Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F). Quadrant markers were set based on control antibody staining (Catalog # IC013N). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Entrez Gene IDs
729230 (Human); 12772 (Mouse); 60463 (Rat)
Alternate Names
C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Mouse CCR2 Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Dipeptidase-1 governs renal inflammation during ischemia reperfusion injury
    Authors: A Lau, JJ Rahn, M Chappellaz, H Chung, H Benediktss, D Bihan, A von Mässen, A Linkermann, CN Jenne, SM Robbins, DL Senger, IA Lewis, J Chun, DA Muruve
    Science Advances, 2022;8(5):eabm0142.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The CD200 Regulates Inflammation in Mice Independently of TNF-&alpha Production
    Authors: K Tonecka, A Braniewska, Z Pilch, Z Sas, M Skorzynski, E Manuali, TP Rygiel
    International Journal of Molecular Sciences, 2021;22(10):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Liver metastasis restrains immunotherapy efficacy via macrophage-mediated T cell elimination
    Authors: J Yu, MD Green, S Li, Y Sun, SN Journey, JE Choi, SM Rizvi, A Qin, JJ Waninger, X Lang, Z Chopra, I El Naqa, J Zhou, Y Bian, L Jiang, A Tezel, J Skvarce, RK Achar, M Sitto, BS Rosen, F Su, SP Narayanan, X Cao, S Wei, W Szeliga, L Vatan, C Mayo, MA Morgan, CA Schonewolf, K Cuneo, I Kryczek, VT Ma, CD Lao, TS Lawrence, N Ramnath, F Wen, AM Chinnaiyan, M Cieslik, A Alva, W Zou
    Nature Medicine, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization
    Authors: J Lian, AJ Ozga, CL Sokol, AD Luster
    Cell Rep, 2020;31(8):107679.
    Species: Mouse
    Sample Types:
    Applications: IHC
  5. Sex-specific adipose tissue imprinting of regulatory T cells
    Authors: A Vasanthaku, D Chisanga, J Blume, R Gloury, K Britt, DC Henstridge, Y Zhan, SV Torres, S Liene, N Collins, E Cao, T Sidwell, C Li, RG Spallanzan, Y Liao, PA Beavis, T Gebhardt, N Trevaskis, SL Nutt, JD Zajac, RA Davey, MA Febbraio, D Mathis, W Shi, A Kallies
    Nature, 2020;0(0):.
    Species: Mouse
    Sample Types: Cells
    Applications: Flow Cytometry
  6. Loss of IL-10 Promotes Differentiation of Microglia to a M1 Phenotype
    Authors: B Laffer, D Bauer, S Wasmuth, M Busch, TV Jalilvand, S Thanos, G Meyer Zu H, K Loser, T Langmann, A Heiligenha, M Kasper
    Front Cell Neurosci, 2019;13(0):430.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Dectin-2-induced CCL2 production in tissue-resident macrophages ignites cardiac arteritis
    Authors: C Miyabe, Y Miyabe, L Moreno, J Lian, RA Rahimi, NN Miura, N Ohno, Y Iwakura, T Kawakami, AD Luster
    J. Clin. Invest., 2019;130(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Robust Control of a Brain-Persisting Parasite through MHC I Presentation by Infected Neurons
    Authors: A Salvioni, M Belloy, A Lebourg, E Bassot, V Cantaloube, V Vasseur, S Blanié, RS Liblau, E Suberbiell, EA Robey, N Blanchard
    Cell Rep, 2019;27(11):3254-3268.e8.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. HIF-1? is a key regulator in potentiating suppressor activity and limiting the microbicidal capacity of MDSC-like cells during visceral leishmaniasis
    Authors: A Hammami, BM Abidin, T Charpentie, A Fabié, AP Duguay, KM Heinonen, S Stäger
    PLoS Pathog., 2017;13(9):e1006616.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Infection-adapted emergency hematopoiesis promotes visceral leishmaniasis
    Authors: BM Abidin, A Hammami, S Stäger, KM Heinonen
    PLoS Pathog., 2017;13(8):e1006422.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Leishmania promastigotes induce cytokine secretion in macrophages through the degradation of synaptotagmin XI.
    Authors: Arango Duque G, Fukuda M, Turco S, Stager S, Descoteaux A
    J Immunol, 2014;193(5):2363-72.


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Mouse CCR2 Alexa Fluor® 700-conjugated Antibody
By Jin sub Kim on 06/20/2018
Application: Flow Sample Tested: Anti-mouse CCR2 A700 conjugated rat IgG2B antibody,Mouse whole blood Species: Mouse