Mouse CCR2 Alexa Fluor® 750-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.
  • Source
    Monoclonal Rat IgG2B Clone # 475301
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    L1.2 mouse pro-B cell line transfected with mouse CCR2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 750
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F) and either (A) Rat Anti-Mouse/Rat CCR2 Alexa Fluor® 750‑conjugated Monoclonal Antibody (Catalog # FAB5538S) or (B) Rat IgG2B Alexa Fluor 750 Isotype Control (Catalog # IC013S). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR2 in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with mouse CCR2 and eGFP was stained with either (A) Rat Anti-Mouse/Rat CCR2 Alexa Fluor® 750‑conjugated Monoclonal Antibody (Catalog # FAB5538S) or (B) Rat IgG2B Alexa Fluor 750 Isotype Control (Catalog # IC013S). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR2
CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that binds CCL2, CCL7, CCL8, CCL12 (in mouse only) and CCL13. CCR2 is expressed by multiple hematopoietic cells plus, endothelial cells, fibroblasts, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.
  • Entrez Gene IDs:
    729230 (Human); 12772 (Mouse); 60463 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC013S
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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