Mouse CCR2 APC-conjugated Antibody FAB5538A-025: R&D Systems

Mouse CCR2 APC-conjugated Antibody

(19 citations)
(3 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.
  • Source
    Monoclonal Rat IgG2B Clone # 475301
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    L1.2 mouse pro-B cell line transfected with mouse CCR2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR2 in L1.2 Mouse Cell Line Transfected with Mouse CCR2 by Flow Cytometry. L1.2 mouse pro-B cell line transfected with mouse CCR2 was stained with Rat Anti-Mouse CCR2 APC‑conjugated Monoclonal Antibody (Catalog # FAB5538A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR2 in Mouse Splenocytes byFlow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR2 APC‑conjugated Monoclonal Antibody (Catalog # FAB5538A) and Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR2
CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.
  • Entrez Gene IDs:
    729230 (Human); 12772 (Mouse); 60463 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. CCR2(-) and CCR2(+) corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion
    Authors: J Liu, Y Xue, D Dong, C Xiao, C Lin, H Wang, F Song, T Fu, Z Wang, J Chen, H Pan, Y Li, D Cai, Z Li
    Mucosal Immunol, 2017;0(0):.
  2. Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors
    Authors: SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk
    Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Monocyte Trafficking, Engraftment, and Delivery of Nanoparticles and an Exogenous Gene into the Acutely Inflamed Brain Tissue - Evaluations on Monocyte-Based Delivery System for the Central Nervous System
    PLoS ONE, 2016;11(4):e0154022.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Pivotal role for skin trans-endothelial radio-resistant anti-inflammatory macrophages in tissue repair
    Authors: Olga Barreiro
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Flow
  5. Alternatively activated macrophages determine repair of the infarcted adult murine heart
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. SHP-1-dependent macrophage differentiation exacerbates virus-induced myositis.
    Authors: Watson N, Schneider K, Massa P
    J Immunol, 2015;194(6):2796-809.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Vitamin D deficiency decreases survival of bacterial meningoencephalitis in mice.
    Authors: Djukic M, Sostmann N, Bertsch T, Mecke M, Nessler S, Manig A, Hanisch U, Triebel J, Bollheimer L, Sieber C, Nau R
    J Neuroinflammation, 2015;12(0):208.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Flow
  8. RGS1 regulates myeloid cell accumulation in atherosclerosis and aortic aneurysm rupture through altered chemokine signalling.
    Authors: Patel, Jyoti, McNeill, Eileen, Douglas, Gillian, Hale, Ashley B, de Bono, Joseph, Lee, Regent, Iqbal, Asif J, Regan-Komito, Daniel, Stylianou, Elena, Greaves, David R, Channon, Keith M
    Nat Commun, 2015;6(0):6614.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. Donor colonic CD103+ dendritic cells determine the severity of acute graft-versus-host disease.
    Authors: Koyama M, Cheong M, Markey K, Gartlan K, Kuns R, Locke K, Lineburg K, Teal B, Leveque-El Mouttie L, Bunting M, Vuckovic S, Zhang P, Teng M, Varelias A, Tey S, Wockner L, Engwerda C, Smyth M, Belz G, McColl S, MacDonald K, Hill G
    J Exp Med, 2015;212(8):1303-21.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. Intraperitoneal prophylaxis with CpG oligodeoxynucleotides protects neutropenic mice against intracerebral Escherichia coli K1 infection.
    Authors: Ribes S, Meister T, Ott M, Redlich S, Janova H, Hanisch U, Nessler S, Nau R
    J Neuroinflammation, 2014;11(0):14.
  11. The monocyte to macrophage transition in the murine sterile wound.
    Authors: Crane, Meredith, Daley, Jean M, van Houtte, Olivier, Brancato, Samielle, Henry, William, Albina, Jorge E
    PLoS ONE, 2014;9(1):e86660.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  12. Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.
    Authors: Habbeddine M, Verbeke P, Karaz S, Bobe P, Kanellopoulos-Langevin C
    PLoS ONE, 2014;9(9):e107267.
  13. Collective nitric oxide production provides tissue-wide immunity during Leishmania infection.
    Authors: Olekhnovitch R, Ryffel B, Muller A, Bousso P
    J Clin Invest, 2014;124(4):1711-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  14. Preemptive donor apoptotic cell infusions induce IFN-gamma-producing myeloid-derived suppressor cells for cardiac allograft protection.
    Authors: Bryant J, Lerret N, Wang J, Kang H, Tasch J, Zhang Z, Luo X
    J Immunol, 2014;192(12):6092-101.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  15. Transgenic expression of GM-CSF in T cells causes disseminated histiocytosis.
    Authors: van Nieuwenhuijze A, Coghill E, Gray D, Prato S, Metcalf D, Alexander W, Wicks I
    Am J Pathol, 2014;184(1):184-99.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  16. Calcitonin gene-related peptide regulates type IV hypersensitivity through dendritic cell functions.
    Authors: Mikami, Norihisa, Sueda, Kaori, Ogitani, Yusuke, Otani, Ippei, Takatsuji, Miku, Wada, Yasuko, Watanabe, Keiko, Yoshikawa, Rintaro, Nishioka, Satoshi, Hashimoto, Nagisa, Miyagi, Yayoi, Fukada, So-ichir, Yamamoto, Hiroshi, Tsujikawa, Kazutake
    PLoS ONE, 2014;9(1):e86367.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  17. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice.
    Authors: Domingos-Pereira S, Decrausaz L, Derre L, Bobst M, Romero P, Schiller J, Jichlinski P, Nardelli-Haefliger D
    Mucosal Immunol, 2013;6(2):393-404.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  18. Calcitonin gene-related peptide is an important regulator of cutaneous immunity: effect on dendritic cell and T cell functions.
    J. Immunol., 2012;186(12):6886-93.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  19. HMGB1 promotes recruitment of inflammatory cells to damaged tissues by forming a complex with CXCL12 and signaling via CXCR4.
    Authors: Schiraldi M, Raucci A, Munoz LM, Livoti E, Celona B, Venereau E, Apuzzo T, De Marchis F, Pedotti M, Bachi A, Thelen M, Varani L, Mellado M, Proudfoot A, Bianchi ME, Uguccioni M
    J. Exp. Med., 2012;209(3):551-63.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 19 Citations
Isotype Controls
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Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 4  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4.7 (Based on 3 reviews)

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We have 3 reviews tested in 1 species: Mouse.
We have 3 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse CCR2 APC-conjugated Antibody FAB5538A
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Excellent
 Flow Mouse Anonymous 05/23/2017
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Image Details
Flow Cytometry Mouse CCR2 APC-conjugated Antibody FAB5538A
Flow Cytometry: Mouse CCR2 APC-conjugated Antibody [FAB5538A]

Summary

ApplicationFlow Cytometry
Sample TestedLung tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsSingle cell suspensions of mouse cells were isolated from the lung tissue and were blocked with Fc block for 30 minutes. Cells were then stained with the APC-conjugated anti-mouse CCR2 antibody (1: 100 in FACS buffer) for 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry.Data was pregated with FSC/SSC --> FSC-A/FSC-W
Flow Cytometry Mouse CCR2 APC-conjugated Antibody FAB5538A
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Excellent
 Flow Mouse Anonymous 09/29/2016
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Flow Cytometry Mouse CCR2 APC-conjugated Antibody FAB5538A
Flow Cytometry: Mouse CCR2 APC-conjugated Antibody [FAB5538A]

Summary

ApplicationFlow Cytometry
Sample TestedActivated mouse CD8 T cell
SpeciesMouse

Other Experimental Details

Other Experimental DetailsCCR2 expression on mouse CD8 T cell in vitro activated or virus-infected lung
  Very Good
 Flow Mouse Anonymous 08/24/2016
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Summary

ApplicationFlow Cytometry
Sample TestedTumor cell lyastes
SpeciesMouse

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