Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 475301
View all CCR2 Primary Antibodies »

Product Specifications

Immunogen

L1.2 mouse pro-B cell line transfected with mouse CCR2

Specificity

Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CCR2 APC‑conjugated Antibody

Detection of CCR2 antibody in L1.2 Mouse Cell Line Transfected with Mouse CCR2 antibody by Flow Cytometry.

Detection of CCR2 in L1.2 Mouse Cell Line Transfected with Mouse CCR2 by Flow Cytometry.

L1.2 mouse pro-B cell line transfected with mouse CCR2 was stained with Rat Anti-Mouse CCR2 APC-conjugated Monoclonal Antibody (Catalog # FAB5538A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR2 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CCR2 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CCR2 APC-conjugated Monoclonal Antibody (Catalog # FAB5538A) and Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR2 by Flow Cytometry

Detection of CCR2 by Flow Cytometry

IFN-gamma and mAb9.2.27 increase cytotoxicity of microglia against GBM. (D-E) Ex vivo purified rat (D) and patient (E) macrophage/microglia from tumor microenvironment were investigated for cytotoxicity against P3-30 tumor pre-incubated or not with mAb9.2.27. Human TAMs were phenotyped for CD45+CD11b+CCR2 expression (E dot plot: CD45+ against CD11b+. E Histogram: isotype control grey histogram, CCR2 black histogram). Lower panel: % lysis of P3-30 tumor by ex vivo TAM from patient GBM following activation with IgG2a isotype control, or mAb9.2.27. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24127551), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCR2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: L1.2 mouse pro-B cell line transfected with mouse CCR2 and mouse splenocytes

Reviewed Applications

Read 3 reviews rated 4.7 using FAB5538A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Gene Symbol

CCR2

Additional CCR2 Products

Product Documents for Mouse CCR2 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCR2 APC‑conjugated Antibody

For research use only

Citations for Mouse CCR2 APC‑conjugated Antibody

Customer Reviews for Mouse CCR2 APC‑conjugated Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Mouse CCR2 APC-conjugated Antibody
    Name: Jim Hsiao
    Application: Flow Cytometry
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 05/23/2017
    Single cell suspensions of mouse cells were isolated from the lung tissue and were blocked with Fc block for 30 minutes. Cells were then stained with the APC-conjugated anti-mouse CCR2 antibody (1: 100 in FACS buffer) for 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry. Data was pregated with FSC/SSC --> FSC-A/FSC-W
    Mouse CCR2 APC‑conjugated Antibody FAB5538A
  • Mouse CCR2 APC-conjugated Antibody
    Name: Kihong Lim
    Application: Flow Cytometry
    Sample Tested: activated mouse CD8 T cell
    Species: Mouse
    Verified Customer | Posted 09/29/2016
    CCR2 expression on mouse CD8 T cell in vitro activated or virus-infected lung
    Mouse CCR2 APC‑conjugated Antibody FAB5538A
  • Mouse CCR2 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Tumor cell lyastes
    Species: Mouse
    Verified Customer | Posted 08/24/2016

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