Mouse CD11b/Integrin alpha M MAb (Clone M1/70)
Mouse CD11b/Integrin alpha M MAb (Clone M1/70) Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Figure 1: Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, Integrin alpha M, B220, Gr-1 (Ly-6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems Catalog # MAB1356; dotted histogram) in bone marrow-progenitor cells after enrichment using the mouse lineage panel and bead depletion.
Preparation and Storage
Background: CD11b/Integrin alpha M
Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.
- Coligan, J.E. et al. in Current Protocols in Immunology, Isolation of Murine Macrophages (1994) eds., John Wiley & Sons, Inc., Volume 3, Supplement 11, 14.1.4.
Citation for Mouse CD11b/Integrin alpha M MAb (Clone M1/70)
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Negative regulation of monocyte adhesion to arterial elastic laminae by signal regulatory protein alpha and Src homology 2 domain-containing protein-tyrosine phosphatase-1.
Authors: Liu SQ, Alkema PK, Tieche C, Tefft BJ, Liu DZ, Li YC, Sumpio BE, Caprini JA, Paniagua M
J. Biol. Chem., 2005;280(47):39294-301.
Sample Types: Whole Cells
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