This antibody reacts with mouse Integrin alpha M, the alpha subunit of MAC-1 integrin. MAC-1, the heterodimer of CD18/CD11b, is also referred to as integrin beta 2/ alpha -M, CR3 and M01. It is a receptor for fibrinogen, factor X, ICAM-1 and complement fragment C3bi.2, 3 MAC-1 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells.1 Cross-reaction of this antibody with human Integrin alpha M has been reported.4, 5
Monoclonal Rat IgG2b Clone # M1/70
This antibody has been used in combination with antibodies to other lineage markers to deplete the cells of major hematopoietic lineages. Each vial contains sufficient antibody to deplete Integrin alpha M+ cells from 1 x 109 bone marrow cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Figure 1: Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, Integrin alpha M, B220, Gr-1 (Ly-6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems Catalog # MAB1356; dotted histogram) in bone marrow-progenitor cells after enrichment using the mouse lineage panel and bead depletion.
Sterile PBS to a final concentration of 0.5 mg/mL.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: CD11b/Integrin alpha M
Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.
Coligan, J.E. et al. in Current Protocols in Immunology, Isolation of Murine Macrophages (1994) eds., John Wiley & Sons, Inc., Volume 3, Supplement 11, 14.1.4.
Entrez Gene IDs:
3684 (Human); 16409 (Mouse)
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide); Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6
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